应用
SIGMAFAST BCIP®/NBT已被用作以下底物：
- 用于免疫印迹的抗小鼠IgG偶联碱性磷酸酶
- 用于原代成骨细胞组织化学染色中碱性磷酸酶
- 骨髓基质细胞碱性磷酸酶染色
重悬
将一片溶于10 ml去离子水中，得到含有BCIP/NBT的即用型缓冲溶液，pH 9.5。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 2
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
R Gonzalez-Castro et al.
Inflammopharmacology, 26(3), 817-827 (2017-11-03)
The main amyloid-beta (Aβ) variants detected in the human brain are full-length Aβ1-40 and Aβ1-42 peptides; however, a significant proportion of AD brain Aβ consists also of N-terminal truncated/modified species. The majority of the previous immunotherapeutic strategies targeted the N-terminal
Nicolas Leventoux et al.
Stem cell research, 47, 101896-101896 (2020-07-14)
Amyotrophic Lateral Sclerosis is the most common motor neuron degenerative disease in adults, and TARDBP gene mutations have been reported to be involved in the pathogenesis. We present here how we generated the human induced pluripotent stem cell (hiPSC) line
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
实验方案
5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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