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一般描述
固定在琼脂糖凝胶上的m-氨基苯硼酸(m-APBA)可用于捕获免疫球蛋白，但也适用于非特异性作用分子。m-APBA是一种硼酸亲和基质，主要用于高效亲和纯化单克隆抗体。
应用
m-氨基苯硼酸琼脂糖已用于：
- 作为色谱柱成分，用于纯化大肠杆菌TOP10F细胞
- 与各种细胞的裂解液混合，用于检测聚ADP核糖基化(PARylated)蛋白。
- 辣根过氧化物酶(HRPO)的亲和纯化
- 糖化人血清白蛋白(gHSA)纯化
外形
悬浮于含0.002％醋酸氯己定的水中。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
Monika Kijewska et al.
Molecules (Basel, Switzerland), 25(3) (2020-02-14)
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
Qian Chen et al.
The EMBO journal, 40(2), e104542-e104542 (2020-12-03)
Optimal DNA damage response is associated with ADP-ribosylation of histones. However, the underlying molecular mechanism of DNA damage-induced histone ADP-ribosylation remains elusive. Herein, using unbiased mass spectrometry, we identify that glutamate residue 141 (E141) of variant histone H2AX is ADP-ribosylated
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