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Merck
CN

A4503

牛血清白蛋白 来源于牛血清

cold ethanol fraction, pH 5.2, ≥96%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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化学文摘社编号:
9048-46-8
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
MFCD00130384

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产品名称

牛血清白蛋白 来源于牛血清, cold ethanol fraction, pH 5.2, ≥96%

biological source

bovine

assay

≥96%

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

BT and VSV virus, tested

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Quality Level

300

Gene Information

bovine ... ALB(280717)

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Preparation Note

通常称为 Cohn fraction V；本品采用改进的 Cohn 氏冷乙醇分级分离法制备。
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

Application

  • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
  • It has been used in the cytotoxicity assay buffer.
  • It has been used for the induction of protein overload nephropathy in rats.

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000520689
0000520687
0000520688
0000520687
0000520688

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is Product No. A4503, Albumin from bovine serum, tested and found to be protease-free?

    No. If you need such a grade, we would recommend Product No. A3059.

  4. Is Product No. A4503, Albumin from bovine serum, tested and found to be endotoxin-free?

    No. If you need such a grade, we would recommend Product No. A8806.

  5. Is Product No. A4503, Albumin from bovine serum, of US origin?

    It is typically from animals born and raised in the US. You may confirm this information by downloading the Certificate of Origin (CofO) for any lot of A4503 from our website. From the product detail page, simply type in the lot number in the box below the words Certificate of Origin, and click GO.

  6. Is Product No. A4053, Albumin from bovine serum, sterile? If not, how would recommend sterilizing it?

    This product is not necessarily sterile. Solutions of albumin cannot be autoclaved, so in order to sterilize it we would recommend that it be dissolved in the appropriate medium (water, saline or buffered saline), followed by sterile filtration through a low protein-binding membrane.

  7. Do you offer a kit for conjugating ligands to bovine serum albumin (Product A4503)?

    Yes. It is our Product No. MBK1.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Isabella Marcomini et al.
Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Cytochrome C effect on respiration of heart mitochondria: influence of various factors.
Toleikis A et al.
Bioscience Reports, 25, 387-387 (2005)
Rapamycin worsens renal function and intratubular cast formation in protein overload nephropathy.
Coombes JD et al.
Kidney International, 68, 2599-2599 (2005)
Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
Journal of Immunology, 180, 163-163 (2008)
Rui Zhang et al.
PLoS pathogens, 15(2), e1007329-e1007329 (2019-03-01)
Mycobacterial pathogens are the causative agents of chronic infectious diseases like tuberculosis and leprosy. Autophagy has recently emerged as an innate mechanism for defense against these intracellular pathogens. In vitro studies have shown that mycobacteria escaping from phagosomes into the
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Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

白蛋白在细胞培养中的应用

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能，并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

实验方案

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

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相关内容

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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