生物来源
Nigrospora sphaerica
质量水平
蒸汽压
0.55 hPa ( 20 °C)
形式
liquid
颜色
clear colorless
溶解性
H2O: miscible (completely)
抗生素抗菌谱
neoplastics
viruses
作用机制
DNA synthesis | interferes
enzyme | inhibits
储存温度
−20°C
InChI
1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
InChI key
NOFOAYPPHIUXJR-APNQCZIXSA-N
一般描述
阿非迪霉素(Aphidicolin)是一种四环二萜抗生素，具有抗病毒和抗有丝分裂作用，可用于各种细胞系的细胞周期同步。
应用
阿非迪霉素,来自球黑孢(Nigrospora sphaerica)，可用于烟草BY-2细胞的细胞周期同步。可阻滞细胞进入S期。
生化/生理作用
阿非迪霉素特异性抑制负责DNA复制的DNA聚合酶α。还可抑制植物和酵母的α样DNA聚合酶，但不抑制RNA和蛋白质的合成。阿非迪霉素特异性竞争DNA聚合酶α的dCTP特异性结合位点。阿非迪霉素具有抗病毒和抗有丝分裂作用。可将细胞周期阻滞在S期。已证明其可诱导HeLa细胞凋亡。
包装
1 ml
外形
阿非迪霉素即用型溶液，以1 mg/ml DMSO溶液的形式提供。
其他说明
保存于密闭容器内，置于干燥通风处。储存类别（TRGS 510）：可燃液体
WGK
WGK 1
闪点(°F)
closed cup
闪点(°C)
closed cup
K Marheineke et al.
The Journal of biological chemistry, 276(20), 17092-17100 (2001-03-30)
DNA replication origins are located at random with respect to DNA sequence in Xenopus early embryos and on DNA replicated in Xenopus egg extracts. We have recently shown that origins fire throughout the S phase in Xenopus egg extracts. To
Hui Wang et al.
Cancer research, 77(8), 2148-2160 (2017-02-18)
Overexpression of the EVI1 oncogene is associated typically with aggressive myeloid leukemia, but is also detectable in breast carcinoma where its contributions are unexplored. Analyzing a tissue microarray of 608 breast carcinoma patient specimens, we documented EVI1 overexpression in both
M Oguro et al.
European journal of biochemistry, 97(2), 603-607 (1979-07-01)
The mode of action by aphidicolin on DNA polymerase alpha from the nuclear fraction of sea-urchin blastulae was studied. The inhibition of DNA polymerase alpha by aphidicolin was uncompetive with activated DNA and competitive with the four deoxynucleoside triphosphates using
Anne-Hélène Quélo et al.
Journal of experimental botany, 53(369), 669-675 (2002-03-12)
Endoreduplication is a common process in plants that allows cells to increase their DNA content. In the tobacco cell cultures studied in this work it can be induced by simple hormone deprivation. Mesophyll protoplast-derived cells cultured in the presence of
New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha.
J A Huberman
Cell, 23(3), 647-648 (1981-03-01)
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