推荐产品
生物来源
bovine
质量水平
检测方案
≥98% (agarose gel electrophoresis)
形式
lyophilized powder
分子量
~66 kDa
纯化方式
heat shock fractionation
包装
poly bottle of
来源
USA origin
技术
cell culture | mammalian: suitable
杂质
≤0.005% Fatty acids
缺失
≤5.0%, Loss on drying
pH值(酸碱度)
7
溶解性
water: soluble (40 mg/ml)
适用性
suitable for ELISA applications (as diluent)
UniProt登记号
异质活性
BT Virus, none detected
VSV Virus, none detected
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.
应用
Bovine Serum Albumin has been used:
Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.
- to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
- as a constituent in the preparation of albumin droplets
- as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
- as a standard protein to evaluate metal oxide adsorption isotherms
Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特点和优势
- Heat shock fractionated
- Essentially fatty acid-free
制备说明
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备。经过进一步纯化以降低脂肪酸含量。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Initial investigation of acoustic droplet vaporization for occlusion in canine kidney
Ultrasound in Medicine & Biology, 36(10), 1691-1703 (2010)
Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type a spermatogonia
Biology of Reproduction, 83(2), 268-276 (2010)
Protein adsorption onto metal oxide materials in white wine model systems
Journal of Food Science, 67(6), 2118-2121 (2002)
The role of inertial cavitation in acoustic droplet vaporization
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 56(5), 1006-1017 (2009)
EMBO molecular medicine, 10(5) (2018-04-18)
The genetic causes of oocyte meiotic deficiency (OMD), a form of primary infertility characterised by the production of immature oocytes, remain largely unexplored. Using whole exome sequencing, we found that 26% of a cohort of 23 subjects with OMD harboured
商品
可用的荧光原位杂交(FISH)过程、试剂和设备。
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
相关内容
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
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