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A3547

无色肽酶来源于细菌

Name: 无色肽酶 来源于细菌
Brand: SIGMA

lyophilized powder, ≥1,000 units/mg solid

别名:

ACP, Lysyl endopeptidase

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About This Item

UNSPSC代码:

12352204

NACRES:

NA.54

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Supelco

90872

丙酮

Sigma-Aldrich

A3422

Achromopeptidase 来源于细菌

Sigma-Aldrich

M9901

变溶菌素来源于球孢链霉菌 ATCC 21553

Supelco

58958

乙酸乙酯

Sigma-Aldrich

SAE0152

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Sigma-Aldrich

SAE0196

Achromopeptidase from bacteria

Supelco

78445

四氢呋喃

Sigma-Aldrich

295108

二氧化碳

Supelco

8.22251

丙酮

Supelco

91237

2-丙醇

生物来源

bacterial (LYSOBACTER SP. BACTERIA M497-1)

质量水平

200

表单

lyophilized powder

比活

≥1,000 units/mg solid

分子量

27 kDa

技术

cell based assay: suitable

适用性

suitable for cell lysis

应用

diagnostic assay manufacturing

运输

dry ice

储存温度

−20°C

应用

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。

生化/生理作用

消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD₆₀₀=0.6，37℃，超过 2 小时。

消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD₆₀₀=0.6，37℃，超过 2 小时。

单位定义

在 pH8.0，37℃下，使用溶壁微球菌（Micropoccus lysodeikticus）悬浮液作为底物，一个单位的酶每分钟每毫升产生 0.001 的 A₆₀₀ 变化（1cm 光程）。

外形

粗粉

象形图

GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P342 + P311

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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分析证书(COA)

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Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.

Shah, et al.

PLoS ONE, 18, e0284424-e0284424 (2023)

Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests.

Georgios Chondrogiannis et al.

Scientific reports, 11(1), 6140-6140 (2021-03-19)

Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP

A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms.

J R Buser et al.

Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)

Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical

Comparison between a chimeric lysin ClyH and other enzymes for extracting DNA to detect methicillin resistant Staphylococcus aureus by quantitative PCR.

Yuanyuan Hu et al.

World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)

Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active

A low-cost, paper-based hybrid capture assay to detect high-risk HPV DNA for cervical cancer screening in low-resource settings.

Chelsey A Smith et al.

Lab on a chip, 23(3), 451-465 (2022-12-24)

Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed

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实验方案

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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安全信息

无色肽酶来源于细菌