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生物来源
bacterial (LYSOBACTER SP. BACTERIA M497-1)
质量水平
形式
lyophilized powder
比活
≥1,000 units/mg solid
分子量
27 kDa
技术
cell based assay: suitable
适用性
suitable for cell lysis
应用
diagnostic assay manufacturing
运输
dry ice
储存温度
−20°C
应用
消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。
生化/生理作用
消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD600 =0.6，37℃，超过 2 小时。
消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。
单位定义
在 pH8.0，37℃下，使用溶壁微球菌（Micropoccus lysodeikticus）悬浮液作为底物，一个单位的酶每分钟每毫升产生 0.001 的 A600 变化（1cm 光程）。
外形
粗粉
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
J R Buser et al.
Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
Yuanyuan Hu et al.
World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active
实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
相关内容
默克提供用于溶解细菌、酵母和昆虫培养物以及植物和哺乳动物细胞培养物和组织样品中蛋白质的蛋白质提取试剂盒、细胞裂解缓冲液和试剂。
Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.
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