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A3547

无色肽酶 来源于细菌

lyophilized powder, ≥1,000 units/mg solid

别名:

ACP, Lysyl endopeptidase

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关于此项目

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1,000 units/mg solid
Biological source:
bacterial (LYSOBACTER SP. BACTERIA M497-1)
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biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Quality Level

200

form

lyophilized powder

specific activity

≥1,000 units/mg solid

mol wt

27 kDa

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Application

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。

Biochem/physiol Actions

消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD600 =0.6，37℃，超过 2 小时。
消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。

Physical form

粗粉

Other Notes

酶用缓冲液悬浮后，在pH 8.0、37 °C下，1单位每分钟可释放0.4 μg溶壁微球菌（浊度法检测，600nm）。

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signalword

Danger

pictograms

Health hazard
GHS08

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000506234
0000394744
0000335811
0000394744
0000335811

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实验方案

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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Cell Lysis Buffers & Protein Extraction Reagents

Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.

全细胞裂解试剂_增强剂_蛋白质样品制备-默克生命科学

默克提供用于溶解细菌、酵母和昆虫培养物以及植物和哺乳动物细胞培养物和组织样品中蛋白质的蛋白质提取试剂盒、细胞裂解缓冲液和试剂。

Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Yuanyuan Hu et al.
World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active
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全球贸易项目编号

货号GTIN
A3547-100KU04061833361054
A3547-1MU04061833361085
A3547-500KU04061832699547