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安全信息

A3547

Sigma-Aldrich

无色肽酶 来源于细菌

lyophilized powder, ≥1,000 units/mg solid

别名:

ACP, Lysyl endopeptidase

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1 L
CN¥2,414.15
10 L
CN¥7,353.88

CN¥2,414.15

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1 L
CN¥2,414.15
10 L
CN¥7,353.88

About This Item

UNSPSC代码:
12352204
NACRES:
NA.54

CN¥2,414.15

请联系客服了解存货情况
获取大包装报价

生物来源

bacterial (LYSOBACTER SP. BACTERIA M497-1)

表单

lyophilized powder

比活

≥1,000 units/mg solid

分子量

27 kDa

技术

cell based assay: suitable

适用性

suitable for cell lysis

应用

diagnostic assay manufacturing

运输

dry ice

储存温度

−20°C

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Glycerol

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Glycerol

form

liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

mp

20 °C (lit.)

mp

20 °C (lit.)

mp

20 °C (lit.)

mp

20 °C (lit.)

agency

USP/NF

agency

-

agency

-

agency

-

vapor density

3.1 (vs air)

vapor density

3.1 (vs air)

vapor density

3.1 (vs air)

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

vapor pressure

<1 mmHg ( 20 °C)

vapor pressure

<1 mmHg ( 20 °C)

vapor pressure

<1 mmHg ( 20 °C)

应用

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定[1]。

生化/生理作用

消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD600 =0.6，37℃，超过 2 小时。
消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。

单位定义

在 pH8.0，37℃下，使用溶壁微球菌（Micropoccus lysodeikticus）悬浮液作为底物，一个单位的酶每分钟每毫升产生 0.001 的 A600 变化（1cm 光程）。

外形

粗粉

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P342 + P311

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000429881
    0000429881
    0000412968
    0000412968
    0000394744

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    Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
    Shah, et al.
    PLoS ONE, 18, e0284424-e0284424 (2023)
    Georgios Chondrogiannis et al.
    Scientific reports, 11(1), 6140-6140 (2021-03-19)
    Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
    Adebayo Shittu et al.
    Journal of medical microbiology, 53(Pt 1), 51-55 (2003-12-10)
    The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to
    Yuanyuan Hu et al.
    World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
    Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active
    Chelsey A Smith et al.
    Lab on a chip, 23(3), 451-465 (2022-12-24)
    Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
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    实验方案

    Enzymatic Assay of Achromopeptidase

    To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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