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形式
partially purified powder
质量水平
比活
≥20,000 units/mg solid
分子量
27 kDa
异质活性
Collagenase, present
储存温度
−20°C
应用
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
生化/生理作用
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
单位定义
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).
外形
Partially purified powder.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
Near real-time inhibitive assay for heavy metals using achromopeptidase
Shukor MY, et al.
Indian Journal of Biotechnology, 13, 398-403 (2014)
Achromopeptidase for lysis of anaerobic gram-positive cocci.
Ezaki T and Suzuki S
Journal of Clinical Microbiology, 16(5), 844-846 (1982)
Metagenomic analysis of an ecological wastewater treatment plant?s microbial communities and their potential to metabolize pharmaceuticals
Balcom IN, et al.
F1000Research, 5, 844-846 (2016)
Microbial ecosystem analysis in root canal infections refractory to endodontic treatment
Henriques LC, et al.
Journal of Endodontics, 42(8), 1239-1245 (2016)
High Intraspecific Genetic Diversity of Nocardia brasiliensis, a Pathogen Responsible for Cutaneous Nocardiosis Found in France: Phylogenetic Relationships by Using sod and hsp65 Genes
Kosova-Maali D, et al.
BioMed Research International, 2018 (2018)
实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
相关内容
默克提供用于溶解细菌、酵母和昆虫培养物以及植物和哺乳动物细胞培养物和组织样品中蛋白质的蛋白质提取试剂盒、细胞裂解缓冲液和试剂。
Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.
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