推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 42 kDa
种属反应性
wide range, vertebrates, human, slime mold, amoeba, chicken
增强验证
independent
Learn more about Antibody Enhanced Validation
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
indirect immunofluorescence: 1:40 using chicken fibroblasts
western blot: 1:100 using chicken gizzard extract
UniProt登记号
应用
research pathology
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
相关类别
一般描述
ACTA1（肌动蛋白，α 1）基因位于人染色体1q42.13上。该基因编码骨骼肌的细丝成分肌动蛋白 1。
在免疫印迹中，本品可用于定位许多物种的肌动蛋白，范围从人骨骼肌到变形虫。在免疫印迹分析中，本品可识别人或动物组织提取物中的42kDa肌动蛋白条带。
特异性
免疫原
应用
用于研究肌动蛋白结构和功能，并检测肌动蛋白结合蛋白的结合位点。使用肌动蛋白作为上样对照品，通过蛋白质免疫印迹法分析从胚胎小鼠肢体组织产生的蛋白质裂解物。使用以1:8000比例稀释的兔抗肌动蛋白抗体检测肌动蛋白。将兔抗肌动蛋白抗体检测的肌动蛋白用作MCF7细胞裂解物的上样对照。在42 kDa处检测到肌动蛋白。
生化/生理作用
目前确知ACTA1（肌动蛋白，α1）突变可导致细肌丝肌病，如线状体肌病、先天性肌病、肌动蛋白肌病、核内棒状体肌病、帽状肌病和轴空性肌病。在没有任何早期自身免疫性肝病证据的情况下，在原位肝移植中已观察到该基因的上调。编码蛋白质肌动蛋白形成细丝网络并调控细胞形状。它提供细胞强度并促进细胞的主动运动。
外形
储存及稳定性
若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。
其他说明
免责声明
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the molecular weight of actin, Products A2066 and A2547?
Actin has a molecular weight of 42 kDa.
Which type of actin does Product A2066, Anti-Actin antibody produced in rabbit, recognize?
This antibody is a polyclonal antibody. The immunogen was a C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) that is found in all actin sequences.
Does Product A2066, Anti-Actin antibody produced in rabbit, react with my species?
The sequence use to develop this antibody is conserved across many species from amoeba to vertebrates. It should react with most samples.
Are there any special considerations for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections?
For FFPE sections, we recommend using enzymatic unmasking.
Why do I have 3 bands on my western blot when using Product A2066, Anti-Actin antibody produced in rabbit?
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93, 2234-2238 (1996).
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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商品
Loading controls in western blotting application.
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