A0810
内切糖苷酶H 来源于褶皱链霉菌
recombinant, expressed in E. coli, buffered aqueous solution
别名:
β-N-乙酰氨基葡萄糖苷酶 H
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一般描述
糖苷内切酶H或称内切β-N-乙酰氨基葡萄糖苷酶H是一种糖水解酶。它由褶皱链霉菌和其它链霉菌种产生。
生化/生理作用
糖苷内切酶H参与裂解高甘露糖型糖中聚糖链核心中两个N-乙酰氨基葡萄糖（GlcNAc）残基之间的N-连接的聚糖。
外形
溶于含有50 nM NaCl、1 mM EDTA的20mM Tris-HCl，pH 7.5溶液
其他说明
在37℃、pH5.5条件下，一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
High-Level Expression of Endo-
Freeze H H and Kranz C
Current Protocols in Molecular Biology, 0 17(3) (2010)
High-Level Expression of Endo-
Wang F, et al.
Testing, 10(3) (2015)
T Tai et al.
The Journal of biological chemistry, 250(21), 8569-8575 (1975-11-10)
Heterogeneities of the two ovalbumin glycopeptides, (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn, were revealed by borate paper electrophoresis of oligosaccharide alcohols obtained from the glycopeptides by endo-beta-N-acetylglucosaminidase H digestion and NaB3H4 reduction. The structures of the major components of the oligosaccharides were determined
Roger S Zou et al.
Aging, 3(10), 968-984 (2011-10-13)
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form
Isabelle Chantret et al.
PloS one, 5(7), e11734-e11734 (2010-07-30)
During mammalian protein N-glycosylation, 20% of all dolichol-linked oligosaccharides (LLO) appear as free oligosaccharides (fOS) bearing the di-N-acetylchitobiose (fOSGN2), or a single N-acetylglucosamine (fOSGN), moiety at their reducing termini. After sequential trimming by cytosolic endo beta-N-acetylglucosaminidase (ENGase) and Man2c1 mannosidase
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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