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Merck
CN

P5493

Sigma-Aldrich

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

别名:

PBS

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About This Item

MDL编号:
UNSPSC代码:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

等级

BioPerformance Certified
for molecular biology

质量水平

100
200

无菌性

0.2 μm filtered

形式

solution

储存条件

(Tightly closed)

技术

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

杂质

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

颜色

colorless

pH值(酸碱度)

7.2-7.6 (25 °C)

溶解性

water: soluble

痕量阳离子

heavy metals (as Pb): ≤5 ppm

适用性

suitable for
suitable for flow cytometry
suitable for microscopy

应用

advanced drug delivery

异质活性

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES字符串

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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应用

磷酸缓冲盐已可用于:
  • 用作人类牙髓干细胞(DPSCs)的细胞洗液
  • 用于脑解剖标本的灌注研究
  • 作为制备细胞标本用于免疫荧光和免疫细胞化学标记的神经元培养液的洗液
  • 体外 培养和受精过程中作为卵巢和精子的洗涤液

包装

4L 盒装,带无漏泄分液龙头。

其他说明

当稀释到 1× 浓度时,本产品可生成磷酸盐缓冲液浓度为 0.01M、氯化钠浓度为 0.154M 的磷酸盐缓冲盐溶液。溶液 pH 为 7.4。
如需了解生化试剂系列的更多信息,请填写此表

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?

    No, it does not contain calcium and magnesium.

  4. What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?

    As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)

实验方案

Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统,可实现快速的高通量ChIP检测

该96孔3D球体BME细胞侵袭检测试剂盒利用符合3D培养要求的96孔球状体形成平板以及专门的球状体形成ECM来促进细胞的聚集和球状体形成。

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.

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