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P3813

Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

别名:

PBS

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About This Item

MDL编号:
MFCD00131855
UNSPSC代码:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions

质量水平

200

形式

powder

储存条件

dry at room temperature

颜色

white

pH值（酸碱度）

7.4

溶解性

water: soluble

密度

1.19 g/cm3 at 20—25 °C
1.22 g/cm3 at 20—25 °C

应用

diagnostic assay manufacturing
life science and biopharma
sample preparation

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相关类别

一般描述

由于其多功能性和关键特性，磷酸盐缓冲液（PBS）是生物学研究中的基础试剂。其主要属性之一是等渗性，类似于人体的pH值、渗透压和离子浓度。这使PBS没有细胞毒性，令其在细胞生物学实验（如细胞清洗、组织运输以及细胞和试剂稀释）中起关键作用。这些应用确保了细胞活力和生理环境的保持。.

PBS的缓冲能力通过磷酸盐-磷酸平衡得到保持，它为实现最佳酶活力和各种生物学过程提供了至关重要的稳定pH 环境。这一特性使PBS成为试剂制备、蛋白质透析和免疫组织化学的理想选择，确保了一致稳定的实验结果。除了细胞生物学外，PBS也可用于多种领域，包括配制定制ELISA缓冲液，以确保最佳的抗体-抗原相互作用。此外，PBS可用作蛋白A/G 色谱的缓冲液，有助于基于特定抗原结合进行抗体纯化。在血液学和生物化学实验中，PBS被广泛用于清洗细胞和组织。

总之，PBS是生物学和生物化学研究中必不可少的工具，可提供等渗性、缓冲能力、简单性、稳定性。 细胞生物学、试剂制备、蛋白质纯化和各种生物学测试中的多样化应用突出了其在增进科学知识和理解中的重要性。

应用

它已被用于漂洗固定的组织，并为免疫定位过程创造合适的缓冲液环境（1） 它已被用作评估ELISA测试试剂盒性能的稀释剂（2）它已被用作免疫组织化学实验中的缓冲剂（3）。
磷酸盐缓冲液已用于：
  • 免疫荧光实验中的清洗溶液。
  • 悬浮细菌细胞。
  • 固定和免疫染色实验。
  • 在免疫定位过程中清洗固定的组织，创造合适的缓冲液环境
  • 作为评估ELISA测试试剂盒性能的稀释剂
  • 作为免疫组织化学实验中的缓冲液

特点和优势

  • 适用于各种研究应用的优质产品
  • 可用作生物学和生物化学应用的缓冲液成分
  • 易溶于水

包装

锡箔袋包装

重悬

当溶于1升蒸馏水或去离子水时，一小袋的物质将得到0.01 M磷酸盐缓冲盐水（NaCl 0.138 M；KCl - 0.0027 M)； pH 7.4, 25 °C时。

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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  1. Is Product P3813, Phosphate buffered saline, a 1 X PBS solution?

    Product P3813 when prepared as directed yields a 1 X PBS solution.

  2. Does Product P3813, Phosphate buffered saline, contain calcium or magnesium ions?

    There is no calcium nor magnesium added to the powder.

  3. What is the shelf life of Product P3813, Phosphate buffered saline?

    The quality for unopened foil pouches stored at room temperature is guaranteed for one year from the time of receipt. The product is expected to be stable for at least 5 years.

  4. How long can solutions of Product P3813, Phosphate buffered saline, be kept once prepared?

    Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8°C can be expected to be stable for several months.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. How much is in each pouch of Product P3813, Phosphate buffered saline?

    Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet.  This is the standard PBS formulation.

  10. Does Product P3813, Phosphate buffered saline, contain calcium, magnesium or sodium bicarbonate?

    The formulation for this phosphate buffered saline product does not contain calcium, magnesium, or sodium bicarbonate.  Sigma does not assay the product for the presence of these compounds (as impurities). None of these compounds are used in the method of preparation.

  11. Is Product P3813, Phosphate buffered saline, sterile?

    Phosphate buffered saline product number P3813 is not necessarily sterile.

  12. Is Product P3813, Phosphate buffered saline, 1X or 10X?

    Each pouch of phosphate buffered saline product number P3813 can be used to prepare 1 Liter of a 1X PBS.

  13. Is Product P3813, Phosphate buffered saline, tested for RNase or DNase impurities?

    Phosphate buffered saline product number P3813 is not tested for RNase or DNase impurities.

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Howarth FC et al.
The Journal of Physiology, 516, 409-409 (1999)
Jennifer L Matthews et al.
Zebrafish, 15(3), 279-290 (2018-01-26)
Sperm cryopreservation is a highly efficient method for preserving genetic resources. It extends the reproductive period of males and significantly reduces costs normally associated with maintenance of live animal colonies. However, previous zebrafish (Danio rerio) cryopreservation methods have produced variable
Dynamic changes in lymphocyte GRK2 levels in cardiac transplant patients: a biomarker for left ventricular function.
Bonita RE et al.
Clinical and Translational Science, 3, 14-14 (2010)
Development of an internal control for evaluation and standardization of a quantitative PCR assay for detection of Helicobacter pylori in drinking water.
Sen K et al.
Applied and Environmental Microbiology, 73, 7380-7380 (2007)
Megan L Linscott et al.
PloS one, 14(7), e0220530-e0220530 (2019-07-31)
Fibroblast growth factor 8 (FGF8) is a potent morphogen that regulates the ontogenesis of gonadotropin-releasing hormone (GnRH) neurons, which control the hypothalamus-pituitary-gonadal (HPG) axis, and therefore reproductive success. Indeed, FGF8 and FGFR1 deficiency severely compromises vertebrate reproduction in mice and
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