推荐产品
形式
powder
质量水平
溶解性
water: soluble, clear, colorless (1 pouch dissolved in 1L)
应用
cell analysis
diagnostic assay manufacturing
正在寻找类似产品? 访问 产品对比指南
一般描述
磷酸盐缓冲液-Tween®20(PBST)主要用作免疫分析中的洗涤和封闭缓冲液。磷酸缓冲液用于维持稳定的pH 7.4，适合许多生物反应和试验。PBS-T中的盐或生理盐溶液用于提供维持最佳蛋白溶解度和稳定性需要的离子强度。0.05% TWEEN®-20是一种非离子去污剂，用于通过封闭蛋白的非特异性结合位点降低背景信号。PBST缓冲液通过清除不必要物质提高洗涤效率，并在保持靶蛋白完整性的前提下控制非特异性抗体结合位点。
应用
磷酸盐缓冲液用于：
- 蛋白质免疫印迹分析
- 酶联免疫吸附测定(ELISA)
- 端粒FISH (Tel-FISH)和端粒/免疫染色-FISH (Teli-Fish)
- 细胞分离
包装
锡箔袋包装
重悬
一个袋中的内容物溶解于1L蒸馏水或超纯水水中，将得到0.01 M磷酸盐缓冲液、0.138M NaCl、0.0027M KCl、0.05% Tween 20，pH7.4.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
TWEEN is a registered trademark of Croda International PLC
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
Modern Pathology, 22(11), 1423-1423 (2009)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
Kossi Komlan et al.
PLoS neglected tropical diseases, 12(3), e0006312-e0006312 (2018-03-02)
Mass drug administration (MDA) of ivermectin has become the main intervention to control onchocerciasis or "river blindness". In Togo, after many years of MDA, Onchocerca volvulus infection has declined dramatically, and elimination appears achievable, but in certain river basins the
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
实验方案
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门