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一般描述
GenElute 凝胶回收试剂盒可用于从标准或低熔点琼脂糖凝胶中快速纯化线性和质粒DNA片段。该试剂盒还可用于从聚丙烯酰胺凝胶中纯化DNA。其典型DNA回收率高达80%。每个纯化柱至多可从多达3.5 g的琼脂糖凝胶中结合10 μg DNA。
应用
GenElute™ 凝胶提取试剂盒已可用于琼脂糖凝胶DNA片段的纯化。
分离所得的DNA适于测序、PCR、限制性酶切、克隆和标记等各种下游应用。
特点和优势
- 可结合多达10 μg DNA
- 回收率高达80%
- 每柱最多可处理3.5 g
- 兼容溶解于TAE 或TBE 缓冲液之中的标准和低熔点琼脂糖。
原理
GenElute 凝胶回收试剂盒结合了硅胶结合技术和离心或真空柱形式的便利性。感兴趣的DNA 片段通过溶解琼脂糖凝胶的切片而提取出来。Gel Solubilization Solution可溶解在TBE或TAE缓冲液进行的凝胶反应的琼脂糖凝胶切片。在存在Gel Solubilization Solution的情况下，提取所得的DNA片段选择性吸附到硅胶膜上。经过简单的离心或真空洗涤，去除污染物。最后，在Tris 缓冲液中洗脱结合所得的DNA。
GenElute 凝胶回收试剂盒结合了硅胶结合技术和离心或真空柱形式的便利性。感兴趣的DNA片段可从琼脂糖凝胶切片上提取，并使之结合到硅胶膜上。经过简单的离心或真空洗涤，去除污染物。随后洗脱结合所得的DNA。
纯化所得的DNA适于自动化DNA测序、PCR、限制性酶切、克隆和标记等多种下游应用。
纯化所得的DNA适于自动化DNA测序、PCR、限制性酶切、克隆和标记等多种下游应用。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3
靶器官
Respiratory system
补充剂危害
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
K Michael Lee et al.
Yeast (Chichester, England), 22(6), 431-440 (2005-04-26)
The Saccharomyces cerevisiae ADH2 promoter (P(ADH2)) is repressed several hundred-fold in the presence of glucose; transcription is initiated once the glucose in the medium is exhausted. The promoter can thus be utilized for effective regulation of recombinant gene expression in
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Margaret A Owegi et al.
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Noroviruses are the major cause of nonbacterial gastroenteritis in humans. These viruses have remained refractory to detailed molecular studies because of the lack of a reverse genetics system coupled to a permissive cell line for targeted genetic manipulation. There is
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实验方案
The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.
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