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Merck
CN

D8662

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

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别名:
DPBS
MDL编号:
UNSPSC代码:
12352207
eCl@ss:
32129211
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
glucose: no (Dextro)

运输

ambient

储存温度

2-8°C

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一般描述

杜氏磷酸盐缓冲盐水(D-PBS)是一种用于组织培养的平衡盐溶液。

应用

D-PBS是通用的等渗盐水溶液,用于在制备和连续转移过程中洗涤细胞。
杜氏磷酸盐缓冲盐水(D-PBS)已用于:
  • 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
  • 在Caco-2BBe细胞经上皮电阻(TEER)测量后清洗和平衡细胞
  • 清洗细胞以进行蛋白质分离

杜氏磷酸盐缓冲盐水(D-PBS)已用于:
  • 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
  • 在Caco-2BBe细胞经上皮电阻(TEER)测量后清洗和平衡细胞
  • 清洗细胞以进行蛋白质分离

生化/生理作用

杜氏磷酸盐缓冲盐水(D-PBS)有四种主要用途:
  • 为细胞提供正常细胞代谢所必需的水分和一定体积的无机离子
  • 充当灌溉、运输和稀释液体,同时维持细胞内和细胞外渗透平衡
  • 与碳水化合物(如葡萄糖)结合时,为细胞代谢提供主要的能量来源
  • 提供缓冲系统,以维持培养基的生理pH值范围

相关产品

产品编号
说明
价格

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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实验方案

实体瘤以三维(3D)空间构象生长,导致氧和营养物质以及其他物理和化学应力的异质性暴露。为了模拟三维空间构象,在肿瘤研究中普遍使用三 维体外培养模型。

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

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