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Merck
CN
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文件

D4418

Sigma-Aldrich

SIGMAFAST™ 3,3′-二氨基联苯胺片

tablet, To prepare 15 mL

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别名:
DAB, DAB tablets, Immunohistology substrate
CAS号:
91-95-2
MDL编号:
MFCD00007725
UNSPSC代码:
12352204
PubChem化学物质编号:
24893872
NACRES:
NA.83

质量水平

200

形式

tablet

溶解性

water: 1 tablet/5 mL

储存温度

−20°C

SMILES字符串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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相关类别

应用

开发用于免疫组织学和免疫印迹，作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物，因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水，封片于树脂介质中，并储存以备将来引用。SIGMAFAST™ DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中

重悬

将每片片剂溶于 15 mL 去离子水中，得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Laura Riva et al.
Pathogens (Basel, Switzerland), 10(2) (2021-02-10)
The hepatitis C virus (HCV) life cycle is a tightly regulated process, during which structural and non-structural proteins cooperate. However, the interplay between HCV proteins during genomic RNA replication and progeny virion assembly is not completely understood. Here, we studied
Ya-Chao Wang et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 980-993 (2016-05-14)
The NMDA antagonist memantine preferentially inhibits extrasynaptic NMDA receptors, which are overactivated upon stroke and thought to disturb neuroplasticity. We hypothesized that memantine enhances post-ischemic neurological recovery, brain remodeling, and plasticity. C57BL6/j mice were exposed to intraluminal middle cerebral artery
Erin N Scully et al.
Behavioural brain research, 331, 151-158 (2017-05-17)
Zebra finches (Taeniopygia guttata) are sexually dimorphic songbirds, not only in appearance but also in vocal production: while males produce both calls and songs, females only produce calls. This dimorphism provides a means to contrast the auditory perception of vocalizations
F Gualtieri et al.
Scientific reports, 9(1), 7129-7129 (2019-05-11)
In the mammalian brain, adult hippocampal neurogenesis (AHN) is suppressed by chronic stress, primarily at the ventral pole of the hippocampus. Based upon anatomy, we hypothesise that the caudal pole of the avian Hippocampal Formation (HF) presents a homologous subregion.
Huiming Yang et al.
Nature communications, 11(1), 2582-2582 (2020-05-24)
Polyglutamine expansion in proteins can cause selective neurodegeneration, although the mechanisms are not fully understood. In Huntington's disease (HD), proteolytic processing generates toxic N-terminal huntingtin (HTT) fragments that preferentially kill striatal neurons. Here, using CRISPR/Cas9 to truncate full-length mutant HTT
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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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