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D0426

Sigma-Aldrich

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

别名:

3,3′-二氨基联苯胺过氧化物酶底物, DAB过氧化物酶底物

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About This Item

UNSPSC代码:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ DAB，含金属增强剂, tablet

形式

tablet

质量水平

200

溶解性

water: 1 tablet/5 mL

储存温度

−20°C

相关类别

一般描述

使用含金属强化剂的SIGMA FAST™ DAB可制备用于检测过氧化物酶活性的活性底物溶液，无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。

应用

含金属强化剂的SIGMAFAST™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节（DRG）神经元和前十字韧带（ACL）细胞中胶原蛋白的免疫组织化学染色。
通过添加CoCl2可以增强DAB反应，并产生更强的耐酒精色斑。

重悬

将每个片剂组合溶于5 mL去离子水中，得到含有DAB和金属强化剂的即用型缓冲溶液。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?

    The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.

  4. What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?

    This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.

  5. How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?

    Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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