产品名称
TRIS硼酸盐– EDTA缓冲剂 溶液, for Northern and Southern blotting, solution
pH
8.6-9.0
grade
Molecular Biology
description
long run, concentrate
product line
BioReagent
form
solution
quality
10× concentrate
ready-to-use solution
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
storage temp.
room temp
Quality Level
Application
适合用作核酸电泳的电泳缓冲液，这可能是Northern和Southern杂交实验的一部分。
General description
TBE（Tris/硼酸/EDTA）缓冲液通常用于核酸电泳。TBE溶液在弱碱性条件下能够有效保持DNA的去质子化、水溶性以及防止DNA降解。该浓缩液可以在使用前轻松稀释至1倍或0.5倍（用分子生物学级水）。
Preparation Note
TBE 10倍浓缩液含1.3M TRIS、450 mM硼酸和25 mM EDTA，溶剂为水。
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
L. Johnston-Dow et al.
Biotechniques, 5, 754-754 (1987)
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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