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相关类别
应用
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer。
生化/生理作用
优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
单位定义
在pH 2.0和37℃条件下,1 U对应于每分钟使280 nm处的吸光度增加0.001的酶量(血红蛋白,货号51290,作为底物)如上所述的15′000吸光度-U相当于 ∼1 Bergmeyer-U。 1 Bergmeyer-U是在pH 2.0和37℃条件下水解1 μmmol乙酰基-L-苯基-3,5-二碘代-L-酪氨酸的酶量
分析说明
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
其他说明
可能会涉及到销售限制
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
动植物源性产品
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