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Merck
CN

63689

2-巯基乙醇

BioUltra, Molecular Biology, ≥99.0% (GC)

别名:

β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇

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关于此项目

线性分子式:
HSCH2CH2OH
化学文摘社编号:
分子量:
78.13
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
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产品名称

2-巯基乙醇, BioUltra, Molecular Biology, ≥99.0% (GC)

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

SMILES string

OCCS

grade

Molecular Biology

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.0% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

RNA extraction: suitable
protein extraction: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.500 (lit.)
n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.114 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.05 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3

storage temp.

2-8°C

Quality Level

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Application

2-巯基乙醇已用于
  • 叶子样品蛋白提取缓冲液中。
  • 作为细胞培养基的补充剂。
  • RNA提取过程中。
BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键,通常用于 SDS-PAGE 的样品缓冲液,浓度为 5%。切割分子间(亚基之间)二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。切割分子内(亚基内)二硫键可使亚基完全变性,从而使每个肽根据其链长迁移,而不受二级结构的影响。

Other Notes

使用胍盐对RNA进行分离。其包括利用还原剂对分子内蛋白质二硫键进行破坏,以增强变性

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

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Lot/Batch Number

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商品

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

实验方案

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

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