乙醇脱氢酶 马

Research Area: Neuroscience
Alcohol dehydrogenase is a zinc metalloprotein that forms five classes of isoenzymes through the dimerization of eight different subunits.

Alcohol Dehydrogenase equine has been used in in vitro alcohol dehydrogenase (Adh) assay.

乙醇脱氢酶催化醇向醛的氧化转化。它具有同二聚体结构，其在 C 端和 N 端催化结构域具有辅酶结合结构域。它的活性位点位于域间裂缝处。NAD+ 在活性位点的结合会导致构象变化，从而形成醇底物的结合位点。

Horse liver alcohol dehydrogenase (HL-ADH) is an enzyme with broad specificity, capable of catalyzing the reversible oxidation of a wide variety of primary and secondary alcohols to form their corresponding aldehydes and ketones. Moreover, alcohol dehydrogenase can oxidize ethanol while simultaneously reducing nicotinamide adenine dinucleotide (NAD+) to NADH. Previous studies have demonstrated that ADH and ALDH variants can influence alcohol dependence. Additionally, the ADH genotype has been linked to lacunar infarction and neuropsychiatric diseases.

在 pH 7.0 和 30°C 下，1 U 相当于每分钟还原 1 μmol 苯甲醛所需的酶量。