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Merck
CN
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文件

47671

Sigma-Aldrich

甲酰胺

BioUltra, for molecular biology, ≥99.5% (T)

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别名:
C1 酰胺, 氨基甲醛
线性分子式:
HCONH2
CAS号:
75-12-7
分子量:
45.04
Beilstein:
505995
EC 号:
200-842-0
MDL编号:
MFCD00007941
UNSPSC代码:
12352111
PubChem化学物质编号:
57651075
NACRES:
NA.25

等级

for molecular biology

质量水平

200

蒸汽密度

1.55 (vs air)

蒸汽压

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

产品线

BioUltra

检测方案

≥99.5% (T)

形式

liquid

自燃温度

932 °F

技术

FISH: suitable
hybridization: suitable
protein extraction: suitable

杂质

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

灼烧残渣

≤0.1%

折射率

n20/D 1.447 (lit.)
n20/D 1.447

pH值（酸碱度）

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

溶解性

H2O: 10 M at 20 °C, clear, colorless

密度

1.134 g/mL at 25 °C (lit.)

痕量阴离子

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

痕量阳离子

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES字符串

NC=O

λ

10 M in H2O

紫外吸收

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

应用

genomic analysis

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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相关类别

一般描述

甲酰胺，也称为甲酰胺醛，是由甲酸衍生出的酰胺化合物。它在生物化学研究中起着至关重要的作用，尤其是在核酸（RNA和DNA）的变性和复性方面。这包括杂交、测序凝胶电泳和电子显微镜等应用。在分子生物学中，BioUltra甲酰胺广泛用于多种应用，如核酸杂交缓冲液、基因指纹图谱、南部印迹杂交和北部印迹杂交，以及YAC筛选。它通常通过降低核酸杂交体的熔解温度来发挥作用，从而便于对其进行操作，并能在测序凝胶中改善变性条件，以获得更稳健的结果。

应用

甲酰胺已用于：
  • 作为用于原位杂交的杂交缓冲液中的组分，作为溶液 X 的组分，用于洗涤载玻片以去除盖玻片
  • 光谱核型分析（SKY）的变性溶液的制备
  • 比较基因组杂交（CGH）载玻片预处理的 1% 甲醛溶液的制备
  • RNA 荧光原位杂交（RNA-FISH）的杂交前缓冲液 I、II、III、IV 和杂交缓冲液 I、II 和 III 的制备
  • 作为生产甲酸酯和维生素的电离溶剂，纸张的软化剂，动物胶和水溶性胶，分子生物学中的变性剂

甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

生化/生理作用

甲酰胺是极性有机和无机化合物的良好溶剂，由于其高介电常数，在益生元过程中具有潜在的催化活性。

特点和优势

  • 用途广泛，适应各种实验室和研究应用
  • BioUltra级试剂，适合分子生物学、细胞生物学和生物化学研究
  • 经检测确认，重金属污染水平低，适用性广
  • 不含DNA酶、RNA酶、切口酶和蛋白酶检测了是否存在微量水平的阴离子和阳离子

其他说明

For additional information on our range of Biochemicals, please complete this form.
甲酰胺变性聚丙烯酰胺胶制备；人染色体原位杂交

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H351,H360D,H373

危险分类

Carc. 2 - Repr. 1B - STOT RE 2 Oral

靶器官

Blood

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the best way to deionize Formamide, Product 47671?

    Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.

  6. Are aqueous solutions of Formamide, Product 47671, stable?

    In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).

  7. What is the molarity of the neat liquid Formamide, Product 47671?

    Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
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