推荐产品
生物来源
human neonatal foreskin or adult skin (normal)
质量水平
包装
pkg of 500,000 cells
制造商/商品名称
Cell Applications, Inc
生长模式
Adherent
核型
2n = 46
形态学
Epidermal
技术
cell culture | mammalian: suitable
相关疾病
allergies; cancer
运输
dry ice
储存温度
−196°C
一般描述
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
细胞系来源
Skin
应用
Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response
组分
Basal Medium containing 10% FBS & 10% DMSO
制备说明
- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
传代培养常规
Please refer to the HEK Culture Protocol.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
实验方案
储存——培养的准备工作——培养HEK——传代培养HEK
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
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