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Merck
CN

00062107

MDCK-II Cell Line canine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

别名:

MDCK Type II Cells, MDCK-2 Cells, MDCKII-WT Cells

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关于此项目

NACRES:
NA.81
UNSPSC Code:
41106514

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产品名称

MDCK-II Cell Line canine, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

biological source

canine kidney

description

Canine Cocker Spaniel Kidney

form

liquid

packaging

tube of 5 μg 00062107-DNA-5UG
pkg of vial of cells 00062107-1VL

growth mode

Adherent

karyotype

Dog

morphology

Polarised epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

Quality Level

100

Biochem/physiol Actions

Canine Cocker Spaniel Kidney
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442)
Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C,

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

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  4. What is the Department of Transportation shipping information for this product?

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Manuel S Brugger et al.
Biosensors & bioelectronics, 173, 112807-112807 (2020-11-23)
A Love-wave based biosensor is introduced for analyzing a standardized wound healing assay by observing cell growth and quantifying cell detachment processes. Utilizing the piezoelectric material LiTaO3 36° XY-cut with a thin SiO2-cover layer, shear horizontal surface acoustic waves (SAW)
D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
Olga Danov et al.
Frontiers in medicine, 7, 571003-571003 (2020-11-27)
Smokers with apparently "healthy" lungs suffer from more severe and frequent viral respiratory infections, but the mechanisms underlying this observation are still unclear. Epithelial cells and dendritic cells (DC) form the first line of defense against inhaled noxes such as

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