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用途
1 mL sufficient for 100 mini-gels
质量水平
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
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浓度
10,000 × in DMSO
环保替代产品分类
储存温度
−20°C
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相关类别
一般描述
SYBR® Green II是用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的一种高度敏感染料。SYBR® Green II对RNA染色没有选择性，但与双链DNA结合时相比较，与RNA结合时确实显示出更高的量子产率。
默克生命科学致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。与溴化乙锭染色的标准用途相比，该产品具有固有的更安全的化学特性。如需更多信息，请参阅可在相关内容中找到的文献。
应用
SYBR® Green II RNA可用于：
- 大肠杆菌(Escherichia coli)核糖核酸(RNA)定量分析
- 在Northern blot印迹分析前，对聚丙烯酰胺凝胶分离的5′-ETS rRNA进行染色
- 染色甲醛琼脂糖凝胶，使RNA条带显色
特点和优势
- 用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的超灵敏染色剂
- 497 nm下激发最大，254nm处还有次级激发峰
- SYBR®Green II染色RNA的荧光发射波长为520 nm
- 使用SYBR Green II对琼脂糖/甲醛凝胶进行染色不会干扰RNA转移至细胞膜或Northern印迹分析中的后续杂交，只要预杂交和杂交缓冲液内包含0.1%-0.3% SDS用于去除染料。
- 适合类病毒RNA和细胞多拷贝RNA检测
- 在单链构象多态性(SSCP)分析等要求极高灵敏度的检测方法中，灵敏度超过溴化乙锭
法律信息
SYBR is a registered trademark of Life Technologies
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Benjamin Lau et al.
Molecular cell, 81(2), 293-303 (2020-12-17)
Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension
Human ??defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases
S. Vardar-Sengul
Journal of Periodontal Research (2007)
Michael Richmond et al.
Biochemistry, 50(12), 2298-2312 (2011-02-09)
In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence
L G Lee et al.
Cytometry, 7(6), 508-517 (1986-11-01)
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was
Dyes for in vitro detection of nucleic acids and proteins.
BioProbes, 20, 12-13 (1994)
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