产品线
pHast Pack™
形式
powder
pH值（酸碱度）
8.3
适用性
suitable for gel electrophoresis
异质活性
DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected
SMILES字符串
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
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一般描述
Tris-Borate-EDTA (TBE) buffer is a commonly used running buffer for nucleic gel electrophoresis with a pH range from 8.1-8.5 (25 °C). TBE buffer has a higher buffer capacity than TAE buffer and has a higher resolution for DNA fragments <1500 bp. Borate in TBE buffer acts as an enzyme inhibitor.
应用
Suitable as a running and gel preparation buffer in:
- DNA agarose gel electrophoresis (fragment <1500 bp)
- Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp)
- Denaturing gradient gel electrophoresis
- Northern and Southern blotting
- DNA automated sequencing gel
- Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution)
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
特点和优势
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Minimize chemical exposure to toxic chemicals
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包装
Foil pouches
制备说明
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。
重悬
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 °C to 40 °C.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险声明
危险分类
Repr. 1B
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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