P4864
碘化丙啶 溶液
≥94% purity, solution (1.0 mg/ml in water)
别名:
3,8-二氨基-5-(二乙氨基丙基)-6-苯基苯甲酸碘化丙啶, 碘化丙啶
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产品名称
碘化丙啶 溶液,
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
assay
≥94%
form
liquid
technique(s)
titration: suitable
color
faint orange to very dark orange, and Faint Red to Very Dark Red and Faint Purple to Very Dark Purple and Orange-Red and Red-Orange
solubility
water: 1 mg/mL
εmax
5400-6400 at 491-495 nm in phosphate buffer
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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Application
- 染色DNA
- 通过活力染色法研究胰岛活力
- 可视化间充质干细胞片中的F-肌动蛋白和细胞核
General description
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Propidium Iodide, Product P4864, stain viable or non-viable cells?
Propidium Iodide does not generally enter living cells, However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).
What are the excitation and emission maxima for Propidium Iodide, Product P4864?
Excitation maximum is at 493 nm in water. Emission maximum is at 636 nm in water
How is Propidium Iodide, Product P4864, used for Flow Cytometry?
Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
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