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E7889

Sigma-Aldrich

乙二胺四乙酸 二钠盐 溶液

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

别名:

EDTA 二钠盐, EDTA-Na2, 亚乙基二次氮基四乙酸 二钠盐

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About This Item

线性分子式:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS号:
139-33-3
分子量:
336.21
Beilstein:
3822669
MDL编号:
MFCD00070672
UNSPSC代码:
12352107
PubChem化学物质编号:
24894660
NACRES:
NA.31

等级

for molecular biology

质量水平

300

无菌性

0.2 μm filtered

表单

liquid

反应适用性

reagent type: chelator

浓度

0.5 M in H2O

pH值（酸碱度）

7.5-8.5

适用性

suitable for molecular biology

应用

microbiology

异质活性

DNase, RNase, NICKase and protease, none detected

SMILES字符串

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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相关类别

一般描述

乙二胺四乙酸二钠盐溶液是一种螯合剂，适用于分子生物学应用。
乙二胺四乙酸（EDTA）是一种多元酸。EDTA中带孤对电子的四个羧酸和两个胺基可以螯合钙离子及其他几种金属离子。

应用

乙二胺四乙酸二钠盐溶液已应用于：
  • 盐酸（HCL）中消化蛋白酶-K，以测定角膜样本中DNA的含量
  • 与木瓜蛋白酶结合消化角膜样本，以量化糖胺聚糖（GAG）的含量
  • 预处理中，以剥离生长至80%融合的上皮细胞
  • 制备裂解缓冲液的原液
  • 制备尿素裂解缓冲液

特点和优势

分子生物学级

制备说明

用分子生物学试剂 EDTA (E 5134) 在 18 兆欧水中制备

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

象形图

Health hazard
GHS08

警示用语：

Warning

危险声明

H373

预防措施声明

P260 - P314 - P501

危险分类

STOT RE 2 Inhalation

靶器官

Respiratory Tract

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000403705
0000403705
BCCM6671
0000395957
0000391927

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  1. Can a solution of EDTA disodium salt, Product E7889, be autoclaved?

    Yes, solutions of EDTA may be autoclaved.

  2. What is the pH of E7889, EDTA disodium salt solution?

    E7889 has a pH specification of 7.5 to 8.5 at 25 °C.

  3. How many divalent cations does Product E7889, EDTA disodium salt, complex with?

    EDTA forms 1:1 complex with divalent cations.

  4. What type of biological activity does Product E7889, EDTA disodium salt, exhibit?

    EDTA is a metal ion-dependent enzyme inhibitor. It may be used as a metalloprotease inhibitor at a concentration of 1-10 μm, or as a DNAse I inhibitor at a concentration of 1-10 mM.

  5. At what concentration should I use Product E7889, EDTA disodium salt, as an anticoagulant?

    The optimal concentration of EDTA to be used as an anticoagulant is 1.5 mg of EDTA disodium salt per ml of blood or 1-2 drops of a 2% EDTA solution per ml of blood.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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