用途
mL sufficient for 100 mini-gels
质量水平
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
浓度
10,000 × in DMSO
环保替代产品分类
储存温度
−20°C
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相关类别
一般描述
SYBR® Green II是用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的一种高度敏感染料。SYBR® Green II对RNA染色没有选择性，但与双链DNA结合时相比较，与RNA结合时确实显示出更高的量子产率。
默克生命科学致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。与溴化乙锭染色的标准用途相比，该产品具有固有的更安全的化学特性。如需更多信息，请参阅可在相关内容中找到的文献。
应用
SYBR® Green II RNA可用于：
- 大肠杆菌(Escherichia coli)核糖核酸(RNA)定量分析
- 在Northern blot印迹分析前，对聚丙烯酰胺凝胶分离的5′-ETS rRNA进行染色
- 染色甲醛琼脂糖凝胶，使RNA条带显色
特点和优势
- 用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的超灵敏染色剂
- 497 nm下激发最大，254nm处还有次级激发峰
- SYBR®Green II染色RNA的荧光发射波长为520 nm
- 使用SYBR Green II对琼脂糖/甲醛凝胶进行染色不会干扰RNA转移至细胞膜或Northern印迹分析中的后续杂交，只要预杂交和杂交缓冲液内包含0.1%-0.3% SDS用于去除染料。
- 适合类病毒RNA和细胞多拷贝RNA检测
- 在单链构象多态性(SSCP)分析等要求极高灵敏度的检测方法中，灵敏度超过溴化乙锭
法律信息
SYBR is a registered trademark of Life Technologies
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Michael Richmond et al.
Biochemistry, 50(12), 2298-2312 (2011-02-09)
In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence
Benjamin Lau et al.
Molecular cell, 81(2), 293-303 (2020-12-17)
Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension
Human ??defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases
S. Vardar-Sengul
Journal of Periodontal Research (2007)
Dyes for in vitro detection of nucleic acids and proteins.
BioProbes, 20, 12-13 (1994)
L G Lee et al.
Cytometry, 7(6), 508-517 (1986-11-01)
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was
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