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S9305

SYBR^® Green II RNA 凝胶染色剂

Name: SYBR<SUP>®</SUP> Green II RNA 凝胶染色剂
Brand: SIAL
Price: 2468.27 CNY

10,000 × in DMSO

别名:

RNA gel dye, SYBR^® RNA dye, safer gel stain

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About This Item

CAS号:

172827-25-7

EC 号:

200-664-3

MDL编号:

MFCD00284716

UNSPSC代码:

12171500

NACRES:

NA.52

¥2,468.27

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此商品	194512	109623	58958
822277 乙酸乙酯	194512 乙酸乙酯	109623 乙酸乙酯	58958 乙酸乙酯
application(s) environmental food and beverages industrial qc pharmaceutical	application(s) environmental food and beverages industrial qc pharmaceutical	application(s) environmental food and beverages industrial qc pharmaceutical sample preparation	application(s) cleaning products cosmetics environmental flavors and fragrances food and beverages personal care
assay ≥99.5% (GC)	assay -	assay ≥99.5% (GC)	assay ≥99.9% (GC)
Quality Level 200	Quality Level -	Quality Level 300	Quality Level 200
grade for analytical purposes, for extraction, for synthesis, for cleaning, for preparative purposes	grade for analytical purposes, for cleaning, for extraction, for preparative purposes, for synthesis	grade ACS reagent, for analytical purposes, for extraction, reagent grade	grade analytical standard
solubility 85.3 g/L	solubility -	solubility 85.3 g/L	solubility water: soluble
form liquid	form liquid	form liquid	form -

一般描述

SYBR^® Green II是用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的一种高度敏感染料。SYBR^® Green II对RNA染色没有选择性，但与双链DNA结合时相比较，与RNA结合时确实显示出更高的量子产率。

默克生命科学致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。与溴化乙锭染色的标准用途相比，该产品具有固有的更安全的化学特性。如需更多信息，请参阅可在相关内容中找到的文献。

应用

SYBR^® Green II RNA可用于：

大肠杆菌(Escherichia coli)核糖核酸(RNA)定量分析[1]
在Northern blot印迹分析前，对聚丙烯酰胺凝胶分离的5′-ETS rRNA进行染色[2]
染色甲醛琼脂糖凝胶，使RNA条带显色[3]

特点和优势

用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的超灵敏染色剂
497 nm下激发最大，254nm处还有次级激发峰
SYBR^®Green II染色RNA的荧光发射波长为520 nm
使用SYBR Green II对琼脂糖/甲醛凝胶进行染色不会干扰RNA转移至细胞膜或Northern印迹分析中的后续杂交，只要预杂交和杂交缓冲液内包含0.1%-0.3% SDS用于去除染料。
适合类病毒RNA和细胞多拷贝RNA检测
在单链构象多态性(SSCP)分析等要求极高灵敏度的检测方法中，灵敏度超过溴化乙锭

法律信息

SYBR is a registered trademark of Life Technologies

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说明

价格

01494

Nancy-520, suitable for RT-PCR, ≥95.0% (HPCE)

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.

Benjamin Lau et al.

Molecular cell, 81(2), 293-303 (2020-12-17)

Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension

RapA, Escherichia coli RNA polymerase SWI/SNF subunit-dependent polyadenylation of RNA.

Michael Richmond et al.

Biochemistry, 50(12), 2298-2312 (2011-02-09)

In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence

Thiazole orange: a new dye for reticulocyte analysis.

L G Lee et al.

Cytometry, 7(6), 508-517 (1986-11-01)

The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was

Dyes for in vitro detection of nucleic acids and proteins.

BioProbes, 20, 12-13 (1994)

90S pre-ribosome transformation into the primordial 40S subunit.

Jingdong Cheng et al.

Science (New York, N.Y.), 369(6510), 1470-1476 (2020-09-19)

Production of small ribosomal subunits initially requires the formation of a 90S precursor followed by an enigmatic process of restructuring into the primordial pre-40S subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway

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SYBR^® Green II RNA 凝胶染色剂

10,000 × in DMSO

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