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Merck
CN

93595

Sigma-Aldrich

台盼蓝 溶液

0.4%, for microscopy

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About This Item

经验公式(希尔记法):
C34H24N6Na4O14S4
CAS号:
分子量:
960.81
Beilstein:
4360496
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
MA.02

等级

for microscopy

质量水平

形式

liquid

保质期

limited shelf life, expiry date on the label

浓度

0.4%

颜色

blue to very dark blue

密度

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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一般描述

台盼蓝是一种来源于甲苯胺的四磺化阴离子亲水性偶氮染料。非渗透性细胞膜染料是一种重要染料,可穿过受损细胞膜但无法穿透完整的细胞膜,从而选择性染色死细胞或无活力细胞。

应用

台盼蓝的应用包括:
  • 它常用于各种细胞类型的活性测试,包括冷冻精子和接触杀真菌药物的主动脉肌细胞等。
  • 在Van Gieson过程中作为胶原蛋白染色剂
  • 染色淀粉样蛋白,台盼蓝可作为Puchtler-Bennhold染色法中刚果红的蓝色替代染剂。
  • 用作胚胎学中细胞群的荧光示踪剂
  • 用作肿瘤启动子调节溶酶体膜的渗透性
台盼蓝溶液还被用于细胞毒性测试,以及用于分析确定细胞分裂的活细胞。

生化/生理作用

台盼蓝被大多数活细胞阻挡,但可被带入吞噬细胞和某些其他细胞。

外形

0.4% 溶于 0.81% 氯化钠,0.06% 磷酸氢二钾

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Carc. 1B

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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实验方案

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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