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Merck
CN
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安全信息

62970

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

dialyzed, lyophilized, powder, ~100000 U/mg

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS号:
12650-88-3
EC 号:
3.2.1.17 (BRENDA, IUBMB)
EC 号:
235-747-3
MDL编号:
MFCD00131557
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

chicken egg white

质量水平

100

形式

powder

质量

dialyzed
lyophilized

比活

~100000 U/mg

分子量

single-chain 14.3 kDa
Mr ~14600

技术

cell based assay: suitable

适用性

suitable for cell lysis

UniProt登记号

P00698

应用

cell analysis

储存温度

2-8°C

基因信息

chicken ... LYZ(396218)

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相关类别

应用

来自鸡蛋清的溶菌酶已被用于：
  • 作为小角度中子散射实验 和红外吸收光谱的标准品
  • 在蛋白质结晶实验中，作为细胞裂解缓冲液的组分

酶可分解细菌的细胞壁；用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。

单位定义

1 U对应于在pH7.0和25°C下，将酶在450nm处的吸光度降低0.001（每分钟0.001）所需的酶量[藤黄微球菌（Micrococcus luteus），ATCC 4698，作为底物]

其他说明

适用于细菌细胞壁、粘多糖、粘多肽或几丁质的水解; 乳酸乳球菌亚种cremoris H2细胞壁的可降解性;综述

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
BCCC9899
BCBV6782
BCBP7829V
BCBM6718V
BCBJ3719V

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T Coolbear et al.
Applied and environmental microbiology, 58(10), 3263-3270 (1992-10-01)
The cell wall-associated proteinase from Lactococcus lactis subsp. cremoris H2 (isolate number 4409) was released from the cells by treatment with lysozyme, even in the presence of 50 mM calcium chloride. Cell lysis during lysozyme treatment was minimal. The proteinase
R.W. Franck
Bioorganic Chemistry, 20, 77-77 (1992)
Structures and interactions among lysozyme proteins below the isoelectric point in presence of divalent ions
Pandit S, et al.
Chemical Physics Letters, 711, 8-14 (2018)
L. Stevens
Comp. Biochem. Physiol., B: Comp. Biochem., 100, 1-1 (1991)
POTRA Domains, Extracellular Lid, and Membrane Composition Modulate the Conformational Stability of the beta Barrel Assembly Factor BamA
Thoma J, et al.
Structure, 26(7), 987-996 (2018)
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