等级
ACS reagent
质量水平
形式
powder
技术
titration: suitable
visual transition interval
3.0-4.6, yellow to blue
mp
273 °C (lit.)
溶解性
ethanol: 0.1%
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key
UDSAIICHUKSCKT-UHFFFAOYSA-N
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一般描述
溴酚蓝在酸性水溶液中以磺化羟基醌的形式存在。又名四溴酚磺酞。溴酚蓝是一种pH指示剂，并且是一种强蓝色染料。 它带有轻微的负电荷并且与DNA同向迁移，方便用户对分子在凝胶中的移动进程进行监测。不同的凝胶组成具有不同的迁移速率。
应用
溴酚蓝可用作DNA、RNA（琼脂糖）和蛋白（聚丙烯酰胺）凝胶电泳中的示踪染料。它被用于为蛋白质印迹分析制备蛋白质样品。溴酚蓝在琼脂糖凝胶中以与300-500 bp DNA大致相同的速率迁移，在蛋白聚丙烯酰胺凝胶中位于缓冲前沿。 还用作血脑屏障的重要检测染料。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Ibrahim Alkatout et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(1), 69-79 (2014-06-21)
The prognosis of pancreatic neuroendocrine tumors is related to size, histology and proliferation rate. However, this stratification needs to be refined further. We conducted a proteome study on insulinomas, a well-defined pancreatic neuroendocrine tumor entity, in order to identify proteins
Stephanie L Smith-Roe et al.
Oncotarget, 6(2), 732-745 (2014-12-30)
SWI/SNF complexes utilize BRG1 (also known as SMARCA4) or BRM (also known as SMARCA2) as alternative catalytic subunits with ATPase activity to remodel chromatin. These chromatin-remodeling complexes are required for mammalian development and are mutated in ~20% of all human
Ákos Kenéz et al.
PloS one, 10(5), e0127208-e0127208 (2015-05-16)
Dairy cows mobilize large amounts of body fat during early lactation to overcome negative energy balance which typically arises in this period. As an adaptation process, adipose tissues of cows undergo extensive remodeling during late pregnancy and early lactation. The
Dana Westphal et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(39), E4076-E4085 (2014-09-18)
The pivotal step on the mitochondrial pathway to apoptosis is permeabilization of the mitochondrial outer membrane (MOM) by oligomers of the B-cell lymphoma-2 (Bcl-2) family members Bak or Bax. However, how they disrupt MOM integrity is unknown. A longstanding model
Sing Ling Chia et al.
Small (Weinheim an der Bergstrasse, Germany), 11(6), 702-712 (2014-10-22)
Our current mechanistic understanding on the effects of engineered nanoparticles (NPs) on cellular physiology is derived mainly from 2D cell culture studies. However, conventional monolayer cell culture may not accurately model the mass transfer gradient that is expected in 3D
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