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Merck
CN

114391

溴酚蓝

ACS reagent

别名:

3′,3″,5′,5″-四溴苯酚磺酞, 溴酚蓝磺酸内酯

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关于此项目

经验公式(希尔记法):
C19H10Br4O5S
化学文摘社编号:
分子量:
669.96
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-086-2
Beilstein/REAXYS Number:
61698
MDL number:
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InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

grade

ACS reagent

form

powder

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubility

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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General description

溴酚蓝在酸性水溶液中以磺化羟基醌的形式存在。又名四溴酚磺酞。溴酚蓝是一种pH指示剂,并且是一种强蓝色染料。 它带有轻微的负电荷并且与DNA同向迁移,方便用户对分子在凝胶中的移动进程进行监测。不同的凝胶组成具有不同的迁移速率。

Application

溴酚蓝可用作DNA、RNA(琼脂糖)和蛋白(聚丙烯酰胺)凝胶电泳中的示踪染料。它被用于为蛋白质印迹分析制备蛋白质样品。溴酚蓝在琼脂糖凝胶中以与300-500 bp DNA大致相同的速率迁移,在蛋白聚丙烯酰胺凝胶中位于缓冲前沿。 还用作血脑屏障的重要检测染料。

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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