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安全信息

XTG9-RO

Roche

X-tremeGENE™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

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About This Item

UNSPSC代码:
41106502
NACRES:
NA.55

等级

for molecular biology

质量水平

100

形式

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包装

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

制造商/商品名称

Roche

技术

transfection: suitable

储存温度

2-8°C

相关类别

一般描述

X-tremeGENE 9 DNA 转染试剂是一种由脂质和其它成分混合而得的专利混合物，溶于80%乙醇中，经0.2 μm滤膜过滤，然后封装于玻璃小瓶中。

应用

X-treme基因™ 9 DNA转染试剂是一种非脂质体多组分试剂，用于各种实验包括细胞分析。由于它的细胞毒性极低，不太需要进行优化，即使是血清存在条件下，在许多种常用细胞系中都有很高转染效率，因此非常适合于所有的细胞分析。

X-treme基因™ 9 DNA转染试剂适合的细胞分析有：
  • 重组蛋白表达功能分析。
  • 代谢通路生理研究。
  • 使用报道基因的调节序列分析。
  • 基因表达分析。
  • 癌症研究分析。
  • 目标评估。

特点和优势

  • 使用毒性极低，转染后细胞存活率高，生成可信任的生理学相关数据。
  • 节省时间，减少操作步骤；只需对X-tremeGENE 9 DNA转染试剂进行简单稀释，再与质粒DNA共同孵育，即可直接向细胞添加反应混合物（有无血清均可）。
  • 对于常用细胞无需进行费时费力的优化工作。

质量

每一批次的X-tremeGENE™ 9 DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE™ 9 DNA转染试剂（试剂DNA比为3:1μl/μg DNA）向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

外形

本产品溶于80%乙醇中并经0.2 μm滤膜过滤。使用次数：使用标准步骤时，在96孔板内，如使用3:1 试剂-DNA比，则1ml X-tremeGENE 9 DNA转染试剂可以进行6,600次转染；如使用2:1 试剂-DNA比，则1ml X-tremeGENE™ 9 DNA转染试剂可进行10,000转染。

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

X-tremeGENE is a trademark of Roche

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象形图

FlameExclamation mark
GHS02,GHS07

警示用语：

Danger

危险声明

H225 - H319

危险分类

Eye Irrit. 2 - Flam. Liq. 2

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

334.4 °F

闪点(°C)

168 °C

法规信息

危险化学品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Jean Guillon et al.
Journal of enzyme inhibition and medicinal chemistry, 18(2), 147-153 (2003-08-29)
The syntthesis of new N-propargyl-3-pyrrol-1-ylindanamine derivatives, analogues of rasagiline, is described in ten steps starting from the corresponding arylaldehydes via the corresponding cis-3-pyrrol-1-ylindanamines. The cis-configuration of some intermediates has been established using X-ray analysis and NOE experiments. The new N-propargyl-3-pyrrol-1-ylindanamine
Daniel F Comiskey et al.
Nucleic acids research, 43(8), 4202-4218 (2015-04-08)
Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2
Birte Zurek et al.
Methods in molecular biology (Clifton, N.J.), 748, 107-119 (2011-06-28)
Nod1 and Nod2 are pattern recognition receptors of the mammalian innate immune system. They respond to bacterial peptidoglycan fragments and are implicated in host defense against a variety of -different bacterial pathogens. Recent studies furthermore support additional functions of these
L C Costantini et al.
Neuroscience, 89(2), 505-513 (1999-03-17)
To investigate the role of neurotrophins in the initial formation of striatal patch versus matrix, the spatial and temporal expression of trkB receptors was examined using immunohistochemistry. Polyclonal antibodies, against the C-terminus or the tyrosine kinase domain, revealed trkB-immunoreactive cells
Leonardo Freire-de-Lima et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17690-17695 (2011-10-19)
The process termed "epithelial-mesenchymal transition" (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps in tumor cell progression and metastasis. Recently, we showed that the expression of some glycosphingolipids (GSLs) is
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实验方案

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ 9 DNA转染试剂实验方案

约转染前24小时接种细胞，确保细胞处于最佳密度（7090 %融合度）。

X-tremeGENE™ HP DNA转染试剂实验方案

约转染前24小时接种转染细胞，确保细胞处于最佳密度（7090 %融合度）。

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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