推荐产品
质量水平
检测方案
>95%
形式
solution
分子量
Mr 817.7
包装
pkg of 3 mL (300 mg)
制造商/商品名称
Roche
技术
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
储存温度
−20°C
一般描述
NBT是一种发色和可溶性物质。它是碱性磷酸酶（AP）的底物，并在其作用下转化为靛蓝/紫色染料。
应用
- 免疫组织细胞化学
- 蛋白质免疫印迹
- Southern印迹
- Northern印迹
- 菌落和噬菌斑杂交
- 原位杂交
外形
该溶液（100 mg/ml）的溶剂为70%二甲基甲酰胺(DMF) (v/v)。NBT溶液的颜色介于黄色和棕色之间，具体取决于所用批次。
注意：颜色不影响试剂的质量或功能。在某些情况下，可能产生沉淀，在37℃下短暂加热即可使沉淀溶于溶液。
注意：颜色不影响试剂的质量或功能。在某些情况下，可能产生沉淀，在37℃下短暂加热即可使沉淀溶于溶液。
制备说明
工作溶液：染色溶液的制备
- 临用前配制新鲜溶液！对于除DIG标记系统以外的所有应用：将50 μl NBT溶液和37,5 μl BCIP加至10 ml 0.1 M Tris-HCl中，pH 9.5 (20 °C)，含0.1 M NaCl和0.05 M MgCl2。
- 对于DIG标记系统应用：将50 μl NBT溶液和37,5 μl BCIP加至10 ml 0.1 M Tris-HCl中，pH 9.5 (20 °C)，含0.1 M NaCl
- 注：请勿在DIG检测缓冲液中加入MgCl2 ，否则在检测过程后膜上可能会产生斑点背景。碱性磷酸酶不需要Mg2+。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Inhalation - Eye Irrit. 2 - Repr. 1B
WGK
WGK 2
闪点(°F)
167.0 °F
闪点(°C)
75 °C
Stefan Dhein
Practical Methods in Cardiovascular Research (2006)
Ewa Pocheć et al.
Acta biochimica Polonica, 62(2), 323-328 (2015-06-23)
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes
Jingwen Niu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(45), 17691-17709 (2013-11-08)
The long-standing doctrine regarding the functional organization of the direct dorsal column (DDC) pathway is the "somatotopic map" model, which suggests that somatosensory afferents are primarily organized by receptive field instead of modality. Using modality-specific genetic tracing, here we show
J Omar Yáñez-Cuna et al.
Genome research, 22(10), 2018-2030 (2012-04-27)
The regulation of gene expression is mediated at the transcriptional level by enhancer regions that are bound by sequence-specific transcription factors (TFs). Recent studies have shown that the in vivo binding sites of single TFs differ between developmental or cellular
Oliva Mota Segura et al.
Journal of neuroscience research, 98(6), 1232-1249 (2020-02-26)
Gap junctions (GJs) are widely distributed in brains across the animal kingdom. To visualize the GJ- coupled networks of two major mechanosensory neurons in the ganglia of medicinal leeches, we injected these cells with the GJ-permeable tracer Neurobiotin. When diffusion
实验方案
存在有多种可与BM Purple（或通常的NBT/BCIP）组合的复染剂，包括FastGreen FCF和Nuclear Fast Red。
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门