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Merck
CN

11243217001

Roche

层粘蛋白

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

别名:

层粘连蛋白 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤基底膜

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关于此项目

化学文摘社编号:
114956-81-9
UNSPSC Code:
12352207
MDL number:
MFCD00081739
NACRES:
NA.54

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产品名称

层粘蛋白, from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

sterility

sterile; sterile-filtered

assay

≥90% (SDS-PAGE)

form

solution

mol wt

900 kDa

packaging

pkg of 1 mg (2 ml)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q61292

storage temp.

−20°C

Quality Level

100

Gene Information

mouse ... Lamb2(16779)

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相关类别

Analysis Note

纯度：≥90% (SDS-PAGE) (作为层粘连蛋白巢蛋白复合物，1:1)

Application

层粘连蛋白已经证明可促进多种细胞的附着和生长；可用于包被培养皿。它已被用于细胞贴壁测定。推荐以1-2μg/cm2用作细胞培养基质。最佳浓度取决于细胞类型以及应用或研究目标。
层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

General description

层粘连蛋白是从小鼠Engelbreth-Holm-Swarm (EHS)肉瘤的层粘连蛋白-巢蛋白复合物中分离得到的。层粘连蛋白是由α、β和γ 链亚基组成的异源三聚体。它是基底膜结构支架所需的糖蛋白。层粘连蛋白支持许多细胞类型的生长和分化，包括上皮细胞、内皮细胞、神经细胞、肌肉细胞和肝细胞。

生物活性：经测试可促进HT-1080细胞的贴壁。
特异性：对大多数哺乳动物细胞有活性。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
小鼠层粘连蛋白由三条多肽链组成（A：400 kD，B1：230 kD和B2：220 kD），通过二硫键连接，并且是糖基化的。

Physical form

溶液，0.5 mg/ml层粘连蛋白，溶剂为0.15 M NaCl，2 mM EDTA，0.05 M Tris-HCl，pH 7.4，无菌
溶液，通过0.2 ?m孔径滤膜过滤

Preparation Note

工作浓度：2至5 μg/cm2
2至5 μg/cm2 ，用于包被细胞培养容器。
储存条件（工作溶液）：2至8°C
未稀释溶液可在2至8°C下稳定保存至少3个月。
溶液应在2至8°C或冰上小心解冻。

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
75215900
74228300
62867700
44741900
37412300

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Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
John P Snow et al.
Neurobiology of disease, 141, 104881-104881 (2020-04-30)
Alternating hemiplegia of childhood (AHC) is a rare neurodevelopmental disease caused by heterozygous de novo missense mutations in the ATP1A3 gene that encodes the neuronal specific α3 subunit of the Na,K-ATPase (NKA) pump. Mechanisms underlying patient episodes including environmental triggers
Elias Theodorou et al.
Genes & development, 23(5), 575-588 (2009-03-10)
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced
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用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

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