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Merck
CN

10102121001

Roche

腺苷脱氨酶(ADA)

from calf intestine

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别名:
ADA, 腺苷脱氨酶
UNSPSC代码:
12352204

生物来源

bovine intestine

质量水平

形式

suspension

比活

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

包装

pkg of 1 mL (10 mg)

制造商/商品名称

Roche

技术

activity assay: suitable

颜色

white

最佳pH

7.0-7.4

溶解性

water: miscible

适用性

suitable for chemiluminescence

NCBI登记号

UniProt登记号

应用

life science and biopharma

异质活性

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

储存温度

2-8°C

基因信息

bovine ... ADA(280712)

一般描述

腺苷氨基水解酶。
腺苷脱氨酶(ADA)是一种锌金属酶。

应用

化学发光腺苷测定。
将腺苷类似物脱氨基形成相应的肌苷类似物
腺苷脱氨酶(ADA)已与其他试剂一起用于脂解的测定。

生化/生理作用

腺苷脱氨酶(ADA)可参与腺苷脱氨基至肌苷,以及2′ 脱氧腺苷脱氨基至2′ -脱氧肌苷的过程。

质量

污染物:<0.01%碱性磷酸酶、AMP-脱氨酶、鸟嘌呤酶和核苷磷酸化酶

外形

悬浮于3.2M硫酸铵溶液中,pH约为6。存在小颗粒是正常现象。在使用前请搅拌沉淀物再继续使用。

其他说明

仅用于生命科学研究。不可用于诊断。

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available

法规信息

动植物源性产品

分析证书(COA)

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Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but

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