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Merck
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文件

SE1M003M00

Millipore

Millipore® Steriflip®真空管顶过滤器

pore size 0.45 μm, PVDF membrane, funnel capacity 50 mL, pack of 25 ea

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别名:
Steriflip®-HV无菌离心管顶部过滤装置
UNSPSC代码:
41104914
eCl@ss:
32031602
NACRES:
NB.22

物料

PVDF membrane
acrylic housing
polypropylene

质量水平

400

无菌性

sterile

产品线

Steriflip®

特点

holdup volume 0.6 mL
hydrophilic

包装

pack of 25 ea

参数

45 °C max. temp.

技术

sterile filtration: suitable

高度

138 mm

容量

50 mL

直径

6.4 cm

过滤器直径

4 cm

过滤面积

7 cm2

漏斗容量

50 mL

基质

Durapore®

孔径

0.45 μm pore size

配件

double lead thread inlet (with vacuum port)
 double lead thread outlet for 50 mL centrifuge tube

运输

ambient

相关类别

应用

仅供研究使用

Steriflip® 过滤器设计用于水溶液（如组织培养基和生物体液）的无菌真空过滤。

  • 组织培养基+/- 添加剂
  • 缓冲液
  • 生物溶液
Steriflip® 过滤器已用于慢病毒生产。

特点和优势

  • 真空助力的密闭系统省却了麻烦的样品转移操作
  • 用任何孔径的尼龙网过滤器，均可从细胞碎屑中分离细胞
  • 特别适于对小批量培养基除菌
  • 可连接到任何标准的 50 mL 离心柱上，不会造成不必要的材料泼洒、时间浪费和风险

法律信息

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

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Laura M Chambers et al.
PloS one, 15(4), e0228511-e0228511 (2020-04-30)
To evaluate intraperitoneal (IP) tumor engraftment, metastasis and growth in a pre-clinical murine epithelial ovarian cancer (EOC) model using both transabdominal ultrasound (TAUS) and bioluminescence in vivo imaging system (IVIS). Ten female C57Bl/6J mice at six weeks of age were
Mateusz Legut et al.
Cell reports, 30(9), 2859-2868 (2020-03-05)
A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9 positioning. Recently
Jiye Liu et al.
Science advances, 7(23) (2021-06-06)
Immunomodulatory drugs (IMiDs) have markedly improved patient outcome in multiple myeloma (MM); however, resistance to IMiDs commonly underlies relapse of disease. Here, we identify that tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) knockdown (KD)/knockout (KO) in MM cells mediates
Xiaojun Lian et al.
Nature protocols, 8(1), 162-175 (2012-12-22)
The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to functional cardiomyocytes in a completely defined, growth factor- and serum-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate temporal application of a glycogen synthase kinase
Jiye Liu et al.
Leukemia, 33(1), 171-180 (2018-07-22)
Immunomodulatory drugs (IMiDs) including lenalidomide and pomalidomide bind cereblon (CRBN) and activate the CRL4CRBN ubiquitin ligase to trigger proteasomal degradation of the essential transcription factors IKZF1 and IKZF3 and multiple myeloma (MM) cytotoxicity. We have shown that CRBN is also
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实验方案

使用Amicon® Pro纯化超滤管去除去垢剂

连续洗滤法清除蛋白或核酸样品中的去垢剂，即在过滤的同时连续补加不含去垢剂的缓冲液，保持样品体积恒定。

Detergent Removal with Amicon® Pro Centrifugal Filters

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

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