Human ES/iPS Neurogenesis Kit

EMD Millipore′s Human ES/iPS Neurogenesis Kit is a robust and user-friendly kit that contains the necessary serum free/chemically defined media and reagents for the rapid production of expandable neural progenitor cells and terminally differentiated neurons from pluripotent human ES and iPS cells. The media contained in this kit rely on both small molecules neural inducers and supplements to generate a highly enriched population of expandable neural progenitors and end stage neurons based on establish protocols (1,2). Neural progenitors are generated 10 days from starting cultures of traditional feeder-based and/or feeder-free cultures of undifferentiated human ES/iPS cells and can be expanded for over 3-5 passages resulting in a minimum 20-fold expansion. Typically, from 1 well of a 6-well plate containing an estimated 500,000 pluripotent human ES/iPS, approximately 5-10 million neural progenitors can be obtained after 3 passages. The resulting neural progenitor cells are multipotent and can be further differentiated to specific neural cell subtypes of interest.

1) Human ES/iPS Neural Induction Medium (Cat. No. SCM110): 125 mL bottle containing optimized medium for the directed induction of human pluripotent cells to expandable neural progenitor cells. Sufficient reagents are provided for six reactions in a 6 well plate format. 2) ENStem-A Neural Expansion Medium (Cat. No. SCM004): 500 mL bottle containing optimized medium and growth factors for expanding neural progenitor cells. 3) Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111): 100 mL bottle containing optimized medium & reagents for the rapid differentiation of neural progenitors to terminal neurons. Sufficient reagents are provided to differentiate 24 reactions in a 24 well format for 2 weeks.

Store at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.