质量水平
种属反应性(根据同源性预测)
mammals
制造商/商品名称
Chemicon®
技术
cell culture | stem cell: suitable
输入
sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
运输
wet ice
一般描述
应用
干细胞研究
Millipore′碱性磷酸酶检测试剂盒(货号SCR004)是一种特异性和灵敏的工具,可通过确定AP活性来对ES细胞分化进行表型评估。
可从Millipore单独购买单克隆抗体TRA-2-49(产品货号MAB4349)和TRA-2-54(产品货号MAB4354),其可用于检测ES细胞表达的肝/骨/肾AP同工酶。 当用于流式细胞术时,这些试剂可以定量评估ES细胞对AP表达的影响[7]。
仅限研究用途,不可用于诊断
制备说明
当存储在2-8º C,试剂盒的组分可在有效期内一直保持稳定。 请勿冷冻或暴露在高温下。到期后,请丢弃所有剩余的试剂。
其他说明
在AMPD缓冲液(pH 9.5;2 mol/L)中的Napthol AS-BI磷酸盐溶液(4 mg/mL)(产品号90234)。一个15 mL瓶。
法律信息
免责声明
警示用语:
Warning
危险声明
危险分类
Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
商品
Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling
实验方案
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
相关内容
We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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