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SCM075

Sigma-Aldrich

ROCK 抑制剂（Y-27632）

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

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别名:
Y-27632
UNSPSC代码:
12352207
eCl@ss:
32160801
NACRES:
NA.75

质量水平

100

形式

liquid

技术

cell culture | mammalian: suitable

运输

dry ice

相关类别

一般描述

ROCK抑制剂（Y-27632）是包括p160Rock（Ki=140nM）和Rock-II在内的Rho相关蛋白激酶（ROCK）的一种选择性ATP竞争性抑制剂。还可作为肌球蛋白磷酸化和平滑肌收缩的激动剂诱导的Ca2+敏化的一种有效抑制剂。用10uM Y-27632处理一小时后可阻断已发生解离的培养的人类胚胎干细胞（hESC）的凋亡，从而在不影响多能性的情况下提高hESC的存活率和克隆效率1。近期有报告表明Y-27632和Accutase（货号SCR005）所组成的消化酶混合配方，能够通过使用常规的慢速冷冻和快速解冻方法提高单个hESC的解冻后存活率

储存及稳定性

在重悬前：维持在-20ºC避光保存2年。在重悬后：在-20ºC避光保存1年

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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