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安全信息

SCC008

Sigma-Aldrich

ReNcell VM人神经祖细胞系

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

别名:

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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About This Item

UNSPSC代码:
41106509
eCl@ss:
32011203
NACRES:
NA.81

生物来源

human

质量水平

100

制造商/商品名称

Chemicon®
ReNcell

技术

cell culture | stem cell: suitable

输入

sample type neural stem cell(s)

运输

liquid nitrogen

相关类别

一般描述

ReNcell VM是一种永生化的人神经祖细胞系，具有易于分化为神经元和神经胶质细胞的能力。 ReNcell VM源自人胎脑的腹侧中脑区域。 通过用v-myc癌基因进行逆转录病毒转导而永生化，该细胞系在层粘连蛋白上以单层快速生长，倍增时间为20-30小时。 核型分析表明，即使经过长时间传代（>45代），ReNcell VM仍可在培养物中保留正常的二倍体核型。 ReNcell VM由ReNeuron Group plc开发，ReNeuron Group plc是一家专门将人体体干细胞用于治疗的生物技术公司。 在ReNeuron Group plc进行的实验中，ReNcell VM可以在体外分化为高水平的人多巴胺能神经元。此外，已证明从ReNcell VM分化的神经元具有电生理活性。 ReNcell VM可用于多种研究应用，例如神经毒性、神经发生、电生理学、神经递质和受体功能的研究。 ReNcell Vm细胞是按照法律和伦理的方式获得的，符合现行的当地知情同意程序。

ReNcell现在用于阿尔茨海默′病的3D细胞模型！
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease.Nature.2014 Nov 13;515(7526):274-8.
（发表在Nature上）
产品来源：人腹侧中脑组织

细胞系描述

神经干细胞

应用

研究类别
干细胞研究

组分

来自人腹侧中脑组织的> 1x106个活ReNcell VM细胞，冷冻保存。 储存在液氮中。

质量

已经验证了每批ReNcell VM细胞的巢蛋白和Sox 2的高水平表达及其自我更新和多谱系分化能力。通过染色体扩散评估，细胞还显示出正常的核型，并且支原体测试为阴性。已对孕妇血液进行了HIV、HTLV、乙型和丙型肝炎筛查。

储存及稳定性

当在推荐的储存条件（液氮）下储存时，ReNcell VM细胞在有效期内保持稳定。 请勿暴露于高温下。 到期后，请丢弃所有剩余的试剂。 我们建议在十代内使用细胞。

其他说明

根据产品文件中详述的“学术使用协议”的条款，本产品预期仅用于销售和销售给学术机构，以供内部学术研究使用。有关任何其他用途的信息，请联系licensing@emdmillipore.com。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

仅供研究使用在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

WGK

WGK 3

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

高风险级别生物产品-Merck

分析证书(COA)

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Chandrasekhar Kothapalli et al.
Biomaterials science, 8(19), 5452-5464 (2020-10-01)
While the mechanotransduction-induced fate of adult neural stem/progenitor cells (NPCs) is relatively known, how substrate stiffness regulates the temporal evolution of the biomechanics and phenotype of developmentally relevant human fetal NPCs (hNPCs) and their mechanosensing pathways remain unknown. Here, we
Jeremy D Hill et al.
Brain, behavior, and immunity, 76, 165-181 (2018-11-23)
New neurons are continuously produced by neural stem cells (NSCs) within the adult hippocampus. Numerous diseases, including major depressive disorder and HIV-1 associated neurocognitive disorder, are associated with decreased rates of adult neurogenesis. A hallmark of these conditions is a
Mehdi Jorfi et al.
Scientific reports, 8(1), 2450-2450 (2018-02-07)
Neurospheroids are commonly used for in vitro disease modeling and drug screening. However, the heterogeneity in size of the neurospheroids mixtures available through current methods limits their utility when employed for basic mechanistic studies of neurodegenerative diseases or screening for
Ahmed Elsherbini et al.
Acta neuropathologica communications, 8(1), 60-60 (2020-04-30)
Amyloid-β (Aβ) associates with extracellular vesicles termed exosomes. It is not clear whether and how exosomes modulate Aβ neurotoxicity in Alzheimer's disease (AD). We show here that brain tissue and serum from the transgenic mouse model of familial AD (5xFAD)
Kurt Farrell et al.
Experimental cell research, 362(1), 159-171 (2017-11-14)
Neural progenitor cell (NPC) fate is influenced by a variety of biological cues elicited from the surrounding microenvironment and recent studies suggest their possible role in pediatric glioblastoma multiforme (GBM) development. Since a few GBM cells also display NPC characteristics
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商品

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

实验方案

Human Neural Stem Cells Differentiation with TrueGel3D®

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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