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PMEF-CF

EmbryoMax® 原代小鼠胚胎成纤维细胞

别名:

CF-1 MEF Feeder Cells, CF-1 MEFs, CF-1 PMEFs, CF1 Mouse Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells

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关于此项目

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
mouse
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biological source

mouse

Quality Level

100

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

EmbryoMax系列PMEF细胞为研究人员提供了ES细胞培养的便捷解决方案，省去了耗时的饲养层细胞分离和制备过程。许多胚胎干细胞培养方案需要使用原代小鼠胚胎成纤维细胞（PMEF）细胞。在这些方案中，ES细胞通常在单层PMEF饲养层细胞上培养。 饲养层细胞在干细胞培养中发挥两个重要作用：它们会向培养基中分泌几种有助于维持其多能性的重要生长因子，并为ES细胞生长提供细胞基质。
原代小鼠胚胎成纤维细胞CF1株经丝裂霉素C处理，可作为小鼠和人ES/IPS细胞的饲养层。

铺板MEF饲养层细胞

程序：

1.在解冻PMEF饲养层细胞之前，用明胶溶液包被平板/烧瓶
2.在37°C水浴中快速解冻PMEF小瓶，并转移至15 mL试管中（已含有10 mL温PMEF饲养层细胞培养基）。轻轻倒置试管以进行分散，并以300 xg离心4–5分钟。
3.去除上清液，将细胞团块重悬于温热的PMEF饲养层细胞培养基中。
4.从平板/烧瓶中取出明胶溶液，并按照小鼠ES方案指南表4.1中推荐的密度等分PMEF饲养层细胞悬液。
5.在37°C和5%CO2下孵育PMEF饲养层细胞。使用鼠标ES方案指南中的图4A、B和C作为正确PMEF密度和
外观估计的指南。糊化平板可使用12–14天。

Biochem/physiol Actions

原代小鼠胚胎成纤维细胞

Packaging

5-6x106 ea

Preparation Note

接收后，小瓶应在-80°C下储存。如果储存时间超过6个月，则在液氮的气相中储存2年（确保盖紧）。

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

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实验方案

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

诱导多能干细胞重编程实验方案

产生人诱导多能干细胞（iPSC）的干细胞重编程实验方案，包括基于病毒和非病毒RNA的方法。

商品

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Shinobu Ueda et al.
PloS one, 3(7), e2800-e2800 (2008-07-31)
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat
Alec R Nickolls et al.
Disease models & mechanisms, 13(6) (2020-05-20)
The basal lamina is a specialized sheet of dense extracellular matrix (ECM) linked to the plasma membrane of specific cell types in their tissue context, which serves as a structural scaffold for organ genesis and maintenance. Disruption of the basal
Transcriptional signature and memory retention of human-induced pluripotent stem cells.
Marchetto, Maria C N, et al.
Testing, 4, e7076-e7076 (2009)

全球贸易项目编号

货号GTIN
PMEF-CF04053252667404