推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
EA10, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... CDKN1A(1026)
一般描述
Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
This Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) is validated for use in FC, Immunoblotting, IF, IP, Paraffin Sections for the detection of p21WAF1 (Ab-1).
应用
Flow Cytometry (2 g/ml or use Cat. No. OP64F; see application references)
Frozen Sections (5 g/ml or use Cat. No. OP64F)
Immunoblotting (1-3 g/ml)
Immunofluorescence (1-5 g/ml or use Cat. No. OP64F)
Immunoprecipitation (2 g/sample)
Paraffin Sections (5 g/ml or use OP64F; heat pre-treatment required; see comments and application references)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
分析说明
Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
其他说明
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. This antibody will immunoprecipitate p21WAF1 but not associated proteins. For immunoblotting applications, use a 0.22 µm filter and visualize by chemiluminescence. For staining paraffin sections, heating the tissue in 10 mM citrate buffer is required (see application references). In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Mingxuan Xia et al.
Cell cycle (Georgetown, Tex.), 7(11), 1604-1612 (2008-06-04)
The p53 tumor suppressor is a powerful growth suppressive and pro-apoptotic molecule frequently inactivated in human cancer. Many tumors overproduce its negative regulator MDM2, a specific p53 ubiquitin ligase and transcriptional inhibitor, to disable p53 function. Therefore, p53 activation by
Vladimir A Shamanin et al.
Molecular and cellular biology, 24(5), 2144-2152 (2004-02-18)
Inactivation of the ARF-p53 tumor suppressor pathway leads to immortalization of murine fibroblasts. The role of this pathway in immortalization of human epithelial cells is not clear. We analyzed the functionality of the p14(ARF)-p53 pathway in human mammary epithelial cells
I Osen et al.
British journal of urology, 81(6), 862-869 (1998-07-17)
To determine the prognostic role of p53, Ki-67 and p21 for patients with muscle-invasive bladder cancer treated with curative intent by radiotherapy. The study included 131 patients (24 women and 107 men, median age 72 years, range 40-86) with transitional
Gernot Hudelist et al.
Breast cancer research and treatment, 86(3), 281-291 (2004-11-30)
cDNA arrays provide a powerful tool to identify gene expression pattern that are potentially associated with tumor invasion and metastasis. However, genes work at the protein level and, since the transcriptional activity of a gene does not necessarily reflect cellular
Karin Hoppe-Seyler et al.
International journal of molecular medicine, 23(3), 415-420 (2009-02-13)
The malononitrilamide FK778 is a novel immunosuppressive agent with antiproliferative activities. To gain insight into the molecular mechanism of FK778-mediated growth inhibition, we analyzed cells which differ in their p53 status and functionality of retinoblastoma protein (pRb). FK778 acted as
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