生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
RAS 10, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... KRAS(3845)
一般描述
可识别SW480和Y1细胞以及乳腺癌组织中的~21 kDa H-、K-和N-Ras蛋白。
该抗Pan-Ras（Ab-3）小鼠mAb（RAS 10）经验证可用于FC、冰冻切片、免疫印迹、IF、IP和石蜡切片，以检测Pan-Ras（Ab-3）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2/0骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体（请参阅应用参考）。可识别~21 kDa Ras蛋白。
免疫原
重组人p21 Ras
应用
流式细胞术分析（2.5 g/ml）
冷冻切片（2.5 g/ml）
免疫印迹（0.1-3 g/ml，化学发光和比色法，请参阅应用参考文献）
免疫荧光（2.5 g/ml，请参阅应用参考文献）
免疫沉淀（1 g/样品）
石蜡切片（2.5 g/ml，需要皂苷预处理）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
分析说明
阳性对照
SW480或Y1细胞或乳腺癌组织
SW480或Y1细胞或乳腺癌组织
其他说明
Rodenhuis, S. et al., 1992.Cancer Res. (Suppl)52, 2665s.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
p21ras的表达水平在不同组织中是可变的。因此，我们建议在免疫印迹分析之前进行浓缩步骤，以获得最佳结果。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
J M English et al.
The Journal of biological chemistry, 274(44), 31588-31592 (1999-10-26)
The activity of the catalytic domain of the orphan MAP kinase ERK5 is increased by Ras but not Raf-1 in cells, which suggests that ERK5 might mediate Raf-independent signaling by Ras. We found that Raf-1 does contribute to Ras activation
M Bosch et al.
The Journal of biological chemistry, 273(34), 22145-22150 (1998-08-15)
One of the major signaling pathways by which extracellular signals induce cell proliferation and differentiation involves the activation of extracellular signal-regulated kinases (ERKs). Because calmodulin is essential for quiescent cells to enter cell cycle, the role of calmodulin on ERK2
A Pelosi et al.
Oncogene, 32(31), 3648-3654 (2012-09-12)
MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression post-transcriptionally, are involved in many complex cellular processes. Several miRNAs are differentially expressed in hematopoietic tissues and play important roles in normal differentiation, but, when aberrantly regulated, contribute to the abnormal
Qing-Fen Li et al.
Cell reports, 24(11), 2869-2882 (2018-09-13)
Cerebrovascular malformations (CVMs) affect approximately 3% of the population, risking hemorrhagic stroke, seizures, and neurological deficits. Recently Ras mutations have been identified in a majority of brain arterio-venous malformations. We generated an endothelial-specific, inducible HRASV12 mouse model, which results in
J M English et al.
The Journal of biological chemistry, 273(7), 3854-3860 (1998-03-28)
Extracellular signal-regulated protein kinase 5 (ERK5) is a recently discovered orphan mitogen-activated protein kinase for which no substrates or strong activators have been described. Two ERK5 chimeras were created as a novel approach to discover its substrates and upstream regulators.
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