推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
18-2, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... PRKDC(5591)
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).
免疫原
DNA-PK purified from HeLa cells
Epitope: within amino acids 1-2713 of DNA-PK
Human
应用
Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 10 mM PBS, 0.2% BSA, pH 7.4.
重悬
For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析说明
Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
其他说明
Kharbanda, S., et al. 1997. Nature386, 732.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Journal of immunology (Baltimore, Md. : 1950), 174(2), 934-941 (2005-01-07)
Activation-induced cytidine deaminase (AID) is required for Ig class switch recombination, a process that introduces DNA double-strand breaks in B cells. We show in this study that AID associates with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promoting cell survival
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TRAIL is an endogenous death receptor ligand also used therapeutically because of its selective proapoptotic activity in cancer cells. In the present study, we examined chromatin alterations induced by TRAIL and show that TRAIL induces a rapid activation of DNA
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Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3' end linked to a tyrosyl moiety. This type of linkage is found at stalled topoisomerase I (Top1)-DNA covalent complexes, and TDP1 has been implicated in the repair of
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DNA double-strand breaks (DSBs) are repaired by nonhomologous end-joining (NHEJ) and homologous recombination (HR). The NHEJ/HR decision is under complex regulation and involves DNA-dependent protein kinase (DNA-PKcs). HR is elevated in DNA-PKcs null cells, but suppressed by DNA-PKcs kinase inhibitors
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