推荐产品
物料
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
质量水平
无菌性
non-sterile
特点
holdup volume> 10 μL
制造商/商品名称
Microcon®
参数
0.5 mL sample volume
技术
DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable
长度
4.50 cm (1.8 in.)
直径
12.3 mm
过滤面积
0.32 cm2
孔径
30 kDa NMWCO
运输
ambient
一般描述
•具有 >95% 的典型回收率,甚至对稀释溶液也是如此
•能逆向旋转,最大限度地回收,甚至对最小的样品也是如此
•能非常方便地将滤出液或浓缩后样品储存在标准微型离心管中
•浓缩因子高达 100X
特征:
•逆转特性使得小体积样品也能达到高回收率(浓缩因子 <20X)
•低结合力 Ultracel 滤膜
•处理速度快
样品应用:
•法医应用中的基因组 DNA 回收
•核酸的浓缩和脱盐(是乙醇沉淀法的一种高回收率替代方法)
•从扩增的 DNA 中去除引物
•简便的滤液回收,得益于平整的滤膜设计
联系
法律信息
储存分类代码
10-13 - German Storage Class 10 to 13
商品
An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
实验方案
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
基于优化的 LC-MS/MS 工作流程,通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
相关内容
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
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