MABT11
抗-ZO-1抗体，克隆R40.76
clone R40.76, from rat
别名:
tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1
产品名称
抗-ZO-1抗体，克隆R40.76, clone R40.76, from rat
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
R40.76, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Tjp1(21872)
Analysis Note
对照
小鼠脑裂解物
小鼠脑裂解物
通过蛋白质印迹法在小鼠脑裂解物中进行评价。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg小鼠脑裂解物中检测到ZO-1。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg小鼠脑裂解物中检测到ZO-1。
Application
免疫荧光分析： 一个先前批次被独立实验室用于IF（D′Angelo Siliciano，1988）。
抗ZO-1抗体，克隆R40.76检测ZO-1水平，&已发布&验证可用于WB、IP、IH。
研究子类别
ECM蛋白
ECM蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
该抗体识别ZO-1。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
紧密连接是蛋白质的复合物，可在上皮细胞和内皮细胞的质膜结构域之间形成细胞间边界。 许多紧密连接相关蛋白是膜相关鸟苷酸激酶(MAGUK)家族的成员，包括闭合蛋白和透明带闭合蛋白家族成员ZO-1、ZO-2和ZO-3。 这些蛋白质被认为具有结构和信号传导作用，并且特征性地由三个蛋白质-蛋白质相互作用模块定义:PDZ结构域、SH3结构域和鸟苷酸激酶(GuK)结构域。ZO-1与ZO-2或ZO-3形成复合物。此外，这些蛋白质还可以在紧密连接处与密封蛋白、闭合蛋白和F-肌动蛋白缔合，在那里它们在肌动蛋白细胞骨架和紧密连接之间提供连接。ZO-1表达在许多乳腺癌细胞系中显著降低。ZO-2和ZO-3在上皮紧密连接处普遍表达，并且与ZO-1（也在心肌细胞的细胞连接处表达）不同，ZO-2在非上皮组织中不表达。
约220 kDa
Immunogen
对应于小鼠ZO-1的纯化蛋白。
表位：未知
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
纯化的大鼠单克隆IgG2aκ，溶于含0.1 M Tris-甘氨酸（pH 7.4，150 mM NaCl）和0.05%叠氮化钠的缓冲液中。
纯化蛋白G
Preparation Note
自接收之日起，在2-8°C下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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