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安全信息

MABS2026

Sigma-Aldrich

Anti-EphA2 Antibody, clone F2-27

clone F2-27, from mouse

别名:

Ephrin type-A receptor 2, EC: 2.7.10.1, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

F2-27, monoclonal

种属反应性

human

包装

antibody small pack of 25 μg

技术

flow cytometry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

靶向翻译后修饰

unmodified

基因信息

human ... EPHA2(1969)

一般描述

Ephrin type-A receptor 2 (UniProt: P29317; also known as EC: 2.7.10.1, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK) is encoded by the EPHA2 (also known as ECK) gene (Gene ID: 1969) in human. Ephrin type-A receptor 2 is a homodimeric single-pass type I membrane protein that is expressed in brain and glioma tissue and in glioma cell lines. It is also highly expressed in tissues that contain a high proportion of epithelial cells. Its levels are shown to be up-regulated by UV irradiation via a TP53-independent, MAPK-dependent mechanism. Ephrin type-A receptor 2 serves as a receptor tyrosine kinase that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. It is involved in the regulation of migration, integrin-mediated adhesion, and in proliferation and differentiation of cells. Ephrin type-A receptor 2 is phosphorylated on tyrosine residues upon binding and activation by EFNA1. Phosphorylation on Tyr 588 and Tyr 594 is reported to be essential for binding VAV2 and VAV3 while phosphorylation on Tyr 735 and Tyr 930 are required for binding PI3-kinase p85 subunit. It is synthesized with a signal peptide (aa 1-23) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 24-537), a short transmembrane domain (aa 538-558), and a cytoplasmic domain (aa 559-976). Two isoforms of Ephrin type-A receptor 2 have been described that are produced by alternative splicing. EphA2 is reported to be overexpressed in skin tumors murine models and its overexpression may represent a compensatory feedback mechanism during tumorigenesis. (Ref.: Guo, H., et al. (2006). Cancer Res. 66(14); 7050-7058).

特异性

Clone F2-27 specifically detects Ephrin type -A receptor 2 in human.

免疫原

KP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.

应用

Anti-EphA2, clone F2-27, Cat. No. MABS2026, is a mouse monoclonal antibody that detects Ephrin type-A receptor 2 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Inhibition Assay.
Inhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).

Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).

Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Research Category
Signaling

质量

Evaluated by Flow Cytometry in PC-3 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected EphA2 in one million PC-3 cells.

目标描述

108.27 kDa calculated.

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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