Anti-SKP1 Antibody, clone 3F3.1

S-phase kinase-associated protein 1 (UniProt P63208; also known as Cyclin-A/CDK2-associated protein p19, OCP-2, OCP-II, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like) is encoded by the SKP1 (also known as EMC19, OCP2, SKP1A, TCEB1L) gene (Gene ID 6500) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Skp1 is one of the three core components of the SCF complex (Skp1, Cullin, F-box) and functions as an adapter that bridges the F-box protein and Cullin (CUL1). SCF complex depends on various F-box proteins for specific recognition and recruitment of substrates. SCF ligase plays an important role in regulating the transitions between G1/S and G2/M phases.

Expected to react with both spliced isoforms of human SKP1 reported by UniProt (P63208).

Epitope: Internal.

GST-tagged recombinant human SKP1 internal fragment.

Anti-SKP1 Antibody, clone 3F3.1 is an antibody against SKP1 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected SKP1 in HUVECs, A431 and HeLa cells.

Research Category
Signaling

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SKP1 in 10 µg of NIH/3T3 cell lysate.

~18 kDa observed. 18.66 kDa (Isoform 1) and 18.06 kDa (Isoform 2) calculated.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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