Anti-Potassium Channel Kv10.1 Antibody, clone 56

Potassium Channel Kv10.1 is also known as Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1 (EAG channel 1, h-eag, or hEAG1) and Voltage-gated potassium channel subunit Kv10.1. Potassium Channel Kv10.1 is a pore-forming (alpha) subunit of voltage-gated, non-inactivating, delayed rectifier potassium channel. Potassium Channel Kv10.1 mediates IK(NI) current in myoblasts, and can be modulated by cAMP as well as subunit assembly. Potassium Channel Kv10.1 is highly expressed in myoblasts at the onset of fusion and brain tissue. It is not found in normal epithelial cells, but has been detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells.

This antibody recognizes the extracellular, C-terminus domain of Potassium Channel Kv10.1. Additionally, this antibody does not cross react with hEag2 (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Epitope: Extracellular domain

Recombinant protein corresponding to the extracellular domain of human Potassium Channel Kv10.1.

Detect Kv10.1 using this mouse monoclonal antibody, Anti-Potassium Channel Kv10.1 Antibody, clone 56 validated for use in IHC, Activity Assay & Immunofluorescence.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Potassium Channel Kv10.1 in human thalamus and cerebellum tissues.

Immunocytochemistry Analysis: A representative lot detected Potassium Channel Kv10.1 in transfected CHO-K1 and HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Activity Assay Analsyis: A representative lot from an independent laboratory inhibited anchorage-independent cancer cell growth in vitro (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.; Chen, Y., et al. (2011). PLoS One. 6(5):e19257.).

Activity Assay Analysis: A representative lot from independent laboratories inhibited Potassium Channel Kv10.1 currents in transfected HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Activity Assay Analysis: A representative lot from an independent laboratory inhibited cellular proliferation and
migration of certain Acute myeloid leukemia cell lines and primary cultured AML cells in vitro (Agarwal, J. R., et al. (2010). Mol Cancer. 9:18.).

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Evaluated by Immunohistochemistry Analysis in rat frontal lobe tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Potassium Channel Kv10.1 in rat frontal lobe tissue.

111 kDa calculated

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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