Anti-CHRNA2 Antibody, clone 5B2.1

Neuronal acetylcholine receptor subunit alpha-2 (UniProt: Q15822; also known as Cholinergic Receptor Nicotinic Alpha 2 Subunit, Acetylcholine Nicotinic Receptor Alpha 2) is encoded by the CHRNA2 gene (Gene ID: 1135) in human. Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. In neuronal AChR alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Cholinergic Receptor Nicotinic Alpha 2 Subunit is a multi-pass membrane protein that is produced with a signal peptide (aa 1-26), which is subsequently cleaved off. It has an extracellular domain (aa 27-264), a cytoplasmic domain (aa 353-502), and four helical transmembrane domains (aa 265-289; 297-315; 331-352; 503-521). Mutations in CHRNA2 gene are known to cause epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering.

Clone 5B2.1 detects human Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2). It targets an epitope within 52 amino acids from the N-terminal region extracellular domain.

Epitope: extracellular domain

GST/His-tagged recombinant fragment corresponding to 52 amino acids from the N-terminal region of human Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2).

Anti-CHRNA2, clone 5B2.1 Antibody, Cat. No. MABN2249, is a highly specific mouse monoclonal antibody that targets Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHRNA2 in human pancreas, tonsil, and skeletal muscle tissue sections.

Research Category
Neuroscience

Evaluated by Western Blotting in CHRNA2 recombinant protein lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected human recombinant Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2).

59.77 kDa calculated.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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